Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | during hypoxia in Y87-1 and Y87-2 cells, a hypoxia-responsive element regulates expression of the cytosine deaminase gene and facilitates the conversion of 5-fluorocytosine to 5-fluorouracil | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
medicine | hypoxia-inducible cytosine deaminase/5-fluorocytosine therapy in combination with radiation therapy shows both a pronounced bystander effect and a radiosensitizing effect under hypoxic conditions | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of the enzyme cloned in a construct comprising an SV40 minimal promoter and nine copies of a hypoxia-responsive element in Y87-1 and Y87-2 cells confering the 5-fluorocytosine hydrolyzing activity to the transformed cells, negative control are Z87 and U-87 MG cells, overview | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a fusion gene consisting of the SV40 minimal promoter, nine copies of a hypoxia-responsive element, and the yeast CD gene | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | Saccharomyces cerevisiae | recombinant enzyme in gene therapy in human glioblastoma cell lines Y87-1 and Y87-2 | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
additional information | Saccharomyces cerevisiae | cytosine deaminase/5-fluorocytosine exposure induces bystander and radiosensitization effects in hypoxic glioblastoma cell lines Y87-1 and Y87-2 in vitro, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
5-fluorocytosine + H2O | recombinant enzyme in gene therapy in human glioblastoma cell lines Y87-1 and Y87-2 | Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
additional information | cytosine deaminase/5-fluorocytosine exposure induces bystander and radiosensitization effects in hypoxic glioblastoma cell lines Y87-1 and Y87-2 in vitro, overview | Saccharomyces cerevisiae | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Saccharomyces cerevisiae |