Application | Comment | Organism |
---|---|---|
medicine | establishment of a hypoxia-dependent prodrug-activating system using the enzyme cloned into a hypoxia-targeted adenoviral vector, overview | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expression in human cervical epithelial adenocarcinoma cell line HeLa, human pancreatic carcinoma cell line MIA PaCa-2, and human colon carcinoma cell lines WiDr and HT29 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | adenovirus-mediated hypoxia-targeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | Escherichia coli | recombinant enzyme encoded in an adenoviral vector expressed in murine cancers or human cancer cell lines for prodrug cancer gene therapy, Ad/5HREp-BCD-mediated cytotoxicity under hypoxic conditions, overview | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
additional information | Escherichia coli | adenovirus-mediated hypoxia-targeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts of murine tumors, e.g. in human cervical epithelial adenocarcinoma cell line HeLa and human pancreatic carcinoma cell line MIA PaCa-2, as well as in human colon carcinoma cell lines WiDr and HT29, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene codA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
5-fluorocytosine + H2O | recombinant enzyme encoded in an adenoviral vector expressed in murine cancers or human cancer cell lines for prodrug cancer gene therapy, Ad/5HREp-BCD-mediated cytotoxicity under hypoxic conditions, overview | Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
additional information | adenovirus-mediated hypoxia-targeting cytosine deaminase gene therapy enhances radiotherapy in tumour xenografts of murine tumors, e.g. in human cervical epithelial adenocarcinoma cell line HeLa and human pancreatic carcinoma cell line MIA PaCa-2, as well as in human colon carcinoma cell lines WiDr and HT29, overview | Escherichia coli | ? | - |
? |