Literature summary for 3.5.4.1 extracted from

O'Brien, T.A.; Tuong, D.T.; Basso, L.M.; McIvor, R.S.; Orchard, P.J.
Coexpression of the uracil phosphoribosyltransferase gene with a chimeric human nerve growth factor receptor/cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosine (2006), Hum. Gene Ther., 17, 518-530.

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Protein Variants

Protein Variants	Comment	Organism
additional information	co-expression of the uracil phosphoribosyltransferase gene with a chimeric truncated human nerve growth factor receptor/cytosine deaminase fusion gene, using a single retroviral vector, augments cytotoxicity of transduced human T cells exposed to 5-fluorocytosine, for positive selection, overview, construct NG/CDiU expressing UPRT and NG/CD, using a bicistronic message, provides the greatest UPRT activity and killing, reducing the lethal dose of 5-FC sufficient to eradicate 90% of cells	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cytosine + H2O	Saccharomyces cerevisiae	-	uracil + NH3	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5-fluorocytosine + H2O	activation of the prodrug, the cytosine deaminase gene is an alternative gene for negative selection producing the toxic metabolite 5-fluorouracil, sensitivity of cells to 5-FU can be further increased by expression of uracil phosphoribosyltransferase, which catalyzes the conversion of 5-FU to 5-fluorouridine monophosphate	Saccharomyces cerevisiae	5-fluorouracil + NH3	-	?
cytosine + H2O	-	Saccharomyces cerevisiae	uracil + NH3	-	?

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Release 2023.1 (January 2023)