Application | Comment | Organism |
---|---|---|
medicine | mutant enzyme with improved activity towards cancer prodrug 5-fluorouracil for suicide gene therapy | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D314A | 11fold increase in KM-value with cytosine, decrease in KM-value with 5-fluorocytosine | Escherichia coli |
F310A | no activity with cytosine | Escherichia coli |
F316A | KM-value with cytosine similar to wild-type, decrease in KM-value with 5-fluorocytosine | Escherichia coli |
G311A | no activity with cytosine | Escherichia coli |
H312A | 3fold increase in KM-value with cytosine | Escherichia coli |
P318A | 4fold increase in KM-value with cytosine | Escherichia coli |
V315A | no activity with cytosine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
cytosine | pH 7.5, 37°C, wild-type | Escherichia coli | |
0.2 | - |
cytosine | pH 7.5, 37°C, mutant F316A | Escherichia coli | |
0.7 | - |
cytosine | pH 7.5, 37°C, mutant H312A | Escherichia coli | |
0.8 | - |
cytosine | pH 7.5, 37°C, mutant P318A | Escherichia coli | |
2.1 | - |
5-fluorocytosine | pH 7.5, 37°C, mutant F316A | Escherichia coli | |
2.2 | - |
cytosine | pH 7.5, 37°C, mutant D314A | Escherichia coli | |
2.8 | - |
5-fluorocytosine | pH 7.5, 37°C, mutant D314A | Escherichia coli | |
3.3 | - |
5-fluorocytosine | pH 7.5, 37°C, wild-type | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | - |
Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
74.3 | - |
5-fluorocytosine | pH 7.5, 37°C, mutant F316A | Escherichia coli | |
75.6 | - |
5-fluorocytosine | pH 7.5, 37°C, wild-type | Escherichia coli | |
104 | - |
cytosine | pH 7.5, 37°C, mutant D314A | Escherichia coli | |
137.4 | - |
5-fluorocytosine | pH 7.5, 37°C, mutant D314A | Escherichia coli | |
165 | - |
cytosine | pH 7.5, 37°C, wild-type | Escherichia coli | |
189 | - |
cytosine | pH 7.5, 37°C, mutant H312A | Escherichia coli | |
1030 | - |
cytosine | pH 7.5, 37°C, mutant P318A | Escherichia coli | |
2286 | - |
cytosine | pH 7.5, 37°C, mutant F316A | Escherichia coli |