Application | Comment | Organism |
---|---|---|
medicine | enzyme is of interest both for antimicrobial drug design and for gene therapy applications against tumors | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
10 mg/ml purified recombinant His-tagged enzyme, vapour phase equilibration against 11-14% PEG 8000, 0.1 M HEPES, pH 7.3-7.7, 0.2 M MgCl2, in hanging drop geometry, flash-cooling in a buffer containing 30% v/v glycerol for cryoprotection, also crystallization of a seleno-methionine derivatized enzyme, X-ray diffraction structure determination and analysis at 1.5 A resolution | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound to the active site, the metal ion coordinates a hydroxyl nucleophile, binding structure, overview | Escherichia coli | |
Zn2+ | enzyme bound, binding structure, overview | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
6 * 48000, (alphabeta)8 barrel structure, crystal structure | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Escherichia coli | enzyme is an important member of the pyrimidine salvage pathway | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P25524 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
cytosine + H2O = uracil + NH3 | substrate binding involves a significant conformational change that sequesters the reaction complex from solvent, active site architecture and substrate binding, catalytic mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | low activity | Escherichia coli | 5-fluorouracil + NH3 | - |
? | |
cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
additional information | enzyme is an important member of the pyrimidine salvage pathway | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 48000, (alphabeta)8 barrel structure, crystal structure | Escherichia coli |
More | stabilized by a unique domain-swapping interaction between enzyme subunits, the active site is located in the mouth of the enzyme barrel | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
CD | - |
Escherichia coli |