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Literature summary for 3.5.4.1 extracted from
- Purification and properties of cytosine deaminase from Aspergillus fumigatus (1991), J. Ferment. Bioeng., 72, 266-269.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Aspergillus fumigatus |  |
| ATP | - |
Aspergillus fumigatus | |
| Cd2+ | - |
Aspergillus fumigatus | |
| Cd2+ | - |
Serratia marcescens | |
| Cu2+ | - |
Aspergillus fumigatus | |
| Cu2+ | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
| Cu2+ | - |
Serratia marcescens | |
| Fe2+ | - |
Aspergillus fumigatus | |
| Hg2+ | - |
Aspergillus fumigatus | |
| Hg2+ | - |
Serratia marcescens | |
| o-phenanthroline | - |
Aspergillus fumigatus | |
| o-phenanthroline | - |
Saccharomyces cerevisiae | |
| p-chloromercuribenzoic acid | - |
Aspergillus fumigatus | |
| Pb2+ | - |
Aspergillus fumigatus | |
| UTP | - |
Aspergillus fumigatus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
cytosine | - |
Aspergillus fumigatus | |
| 6.5 | - |
5-fluorocytosine | - |
Aspergillus fumigatus | |
| 36 | - |
5-Methylcytosine | - |
Aspergillus fumigatus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus fumigatus | - |
- |
| extracellular | strain JH-13 | Arthrobacter sp. | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
gel filtration | Aspergillus fumigatus |
| 32000 | - |
SDS-PAGE | Aspergillus fumigatus |
| 34000 | - |
- |
Saccharomyces cerevisiae |
| 37000 | - |
- |
Arthrobacter sp. |
| 41000 | - |
- |
Saccharomyces cerevisiae |
| 230000 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 580000 | - |
- |
Serratia marcescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytosine + H2O | Salmonella enterica subsp. enterica serovar Typhimurium | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia marcescens | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Arthrobacter sp. | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Aspergillus fumigatus | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Arthrobacter sp. JH-13 | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Aspergillus fumigatus IFO5840 | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arthrobacter sp. | - |
JH-13 | - |
| Arthrobacter sp. JH-13 | - |
JH-13 | - |
| Aspergillus fumigatus | - |
IFO5840 | - |
| Aspergillus fumigatus IFO5840 | - |
IFO5840 | - |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| Serratia marcescens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus fumigatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
- |
Aspergillus fumigatus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 5.5-8.0, stable for at least 24 h | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorocytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Aspergillus fumigatus | 5-fluorouracil + NH3 | - |
? | |
| 5-fluorocytosine + H2O | - |
Aspergillus fumigatus IFO5840 | 5-fluorouracil + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Saccharomyces cerevisiae | thymine + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Aspergillus fumigatus | thymine + NH3 | - |
? | |
| 5-methylcytosine + H2O | - |
Aspergillus fumigatus IFO5840 | thymine + NH3 | - |
? | |
| cytosine + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia marcescens | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Arthrobacter sp. | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Aspergillus fumigatus | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Arthrobacter sp. JH-13 | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Aspergillus fumigatus IFO5840 | uracil + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE | Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
at pH 7 | Aspergillus fumigatus |
| 35 | - |
- |
Aspergillus fumigatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
extremely thermo-unstable, loses more than 85% activity when heated for 10 min at pH 7 | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Saccharomyces cerevisiae |
| 7 | - |
- |
Aspergillus fumigatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8 | - |
Aspergillus fumigatus |
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