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Literature summary for 3.5.4.1 extracted from

  • Katsuragi, T.; Sakai, T.; Tonomura, K.
    Affinity chromatography of cytosine deaminase from Escherichia coli with immobilized pyrimidine compounds (1986), Agric. Biol. Chem., 50, 1713-1719.
No PubMed abstract available

Application

Application Comment Organism
medicine new approach to chemotherapy of cancer, using substrate 5-fluorocytosine orally and enzyme capsules implanted locally, using conversion of product 5-fluorouracil, a potent antineoplastic substance, which is strongly toxic for mammalian cells as chemotherapeutic tool against tumors, enzyme/prodrug gene therapy, artificial gene composed for metastatic colorectal carcinoma Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine + H2O Escherichia coli
-
uracil + NH3
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
K-12 IFO 3301
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.69
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorocytosine + H2O
-
Escherichia coli 5-fluorouracil + NH3
-
?
cytosine + H2O
-
Escherichia coli uracil + NH3
-
?