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Literature summary for 3.5.4.1 extracted from
- Purification of cytosine deaminase from Serratia marcescens (1975), Agric. Biol. Chem., 39, 1623-1629.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Serratia marcescens |  |
| phosphate | - |
Serratia marcescens | |
General Stability
| General Stability | Organism |
|---|---|
| stable in cell-free extracts | Serratia marcescens |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
- |
Saccharomyces cerevisiae |
| 72000 | - |
8 * 72000, SDS-PAGE | Serratia marcescens |
| 580000 | - |
gel filtration | Serratia marcescens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cytosine + H2O | Escherichia coli | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Saccharomyces cerevisiae | - |
uracil + NH3 | - |
? | |
| cytosine + H2O | Serratia marcescens | - |
uracil + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Saccharomyces cerevisiae | - |
baker's yeast | - |
| Serratia marcescens | - |
IFO 3054 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Serratia marcescens |
| partially | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.2 | - |
- |
Serratia marcescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-methylcytosine + H2O | - |
Saccharomyces cerevisiae | thymine + NH3 | - |
? | |
| cytosine + H2O | - |
Escherichia coli | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Saccharomyces cerevisiae | uracil + NH3 | - |
? | |
| cytosine + H2O | - |
Serratia marcescens | uracil + NH3 | - |
? | |
| additional information | 5-methylcytosine, cytidine, 5'-CMP, adenine, adenosine, 5'-AMP, guanine, guanosine, 5'-GMP and GMP are no substrates | Serratia marcescens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 72000, SDS-PAGE | Serratia marcescens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
after treating for 10 min at pH 7, 75% activity remains | Serratia marcescens |
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