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Literature summary for 3.5.3.3 extracted from
- Biochemical and molecular characterization of a novel high activity creatine amidinohydrolase from Arthrobacter nicotianae strain 02181 (2009), Process Biochem., 44, 460-465.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| N3- | 1 mM, 104% of initial activity | Glutamicibacter nicotianae |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Glutamicibacter nicotianae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, no residual activity | Glutamicibacter nicotianae | |
| Fe3+ | 1 mM, 2.2% residual activity | Glutamicibacter nicotianae | |
| Hg2+ | 1 mM, 9.8% residual activity | Glutamicibacter nicotianae | |
| sodium dodecylsulfate | 5 g/l, no residual activity | Glutamicibacter nicotianae | |
| Zn2+ | 1 mM, 44% residual activity | Glutamicibacter nicotianae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 46 | - |
Creatine | pH 7.0, 37°C | Glutamicibacter nicotianae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 104% of initial activity | Glutamicibacter nicotianae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46400 | - |
2 * 46400, SDS-PAGE | Glutamicibacter nicotianae |
| 90000 | - |
PAGE | Glutamicibacter nicotianae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glutamicibacter nicotianae | A7LCN3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Glutamicibacter nicotianae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 124.4 | - |
pH 7.0, 37°C | Glutamicibacter nicotianae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| creatine + H2O | - |
Glutamicibacter nicotianae | sarcosine + urea | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46400, SDS-PAGE | Glutamicibacter nicotianae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Glutamicibacter nicotianae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | - |
Glutamicibacter nicotianae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable below | Glutamicibacter nicotianae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Glutamicibacter nicotianae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Glutamicibacter nicotianae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | - |
Glutamicibacter nicotianae |
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