Protein Variants | Comment | Organism |
---|---|---|
A109V | enhanced thermal stability compared to wild-type enzyme | Pseudomonas putida |
A109V/V355M | enhanced thermal stability compared to wild-type enzyme, contributions of single point mutations to the thermal stability are additive | Pseudomonas putida |
A109V/V355M/V182I | enhanced thermal stability compared to wild-type enzyme, contributions of single point mutations to the thermal stability are additive | Pseudomonas putida |
V182I | no thermal stabilization | Pseudomonas putida |
V355M | enhanced thermal stability compared to wild-type enzyme | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.5 | - |
Creatine | mutant A109V | Pseudomonas putida | |
13.3 | - |
Creatine | wild type enzyme | Pseudomonas putida | |
13.9 | - |
Creatine | mutant V355M | Pseudomonas putida | |
14.7 | - |
Creatine | mutant V182I | Pseudomonas putida | |
15.6 | - |
Creatine | mutant A109V/V355M/V182I | Pseudomonas putida | |
16.1 | - |
Creatine | mutant A109V/V355M | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
2 * 43000, SDS-PAGE | Pseudomonas putida |
92000 | - |
gel filtration | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
wild-type enzyme and mutant enzymes A109V, V355M, V181I, A109V/V355M, A109V/V355M/V182I | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
creatine + H2O | - |
Pseudomonas putida | sarcosine + urea | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Pseudomonas putida |