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Literature summary for 3.5.3.15 extracted from

  • Zendman, A.J.; Raijmakers, R.; Nijenhuis, S.; Vossenaar, E.R.; Tillaart, M.; Chirivi, R.G.; Raats, J.M.; van Venrooij, W.J.; Drijfhout, J.W.; Pruijn, G.J.
    ABAP: antibody-based assay for peptidylarginine deiminase activity (2007), Anal. Biochem., 369, 232-240.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
PAD2 and PAD4 from spleen, expression of N-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Homo sapiens
EDTA
-
Mus musculus
EDTA
-
Oryctolagus cuniculus
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide
-
Homo sapiens
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide
-
Mus musculus
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine amide
-
Oryctolagus cuniculus
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester complete inhibition of recombinant PAD4 at 2 mM Homo sapiens
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester
-
Mus musculus
N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester
-
Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Mus musculus
Ca2+ required Oryctolagus cuniculus
Ca2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protein-L-arginine + H2O Mus musculus
-
protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O Oryctolagus cuniculus
-
protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O Homo sapiens
-
protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O Mus musculus C57BL/6 / 129S
-
protein-L-citrulline + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9Y2J8 PAD2; isozyme PAD2
-
Mus musculus
-
C57BL/6-129S mice
-
Mus musculus C57BL/6 / 129S
-
C57BL/6-129S mice
-
Oryctolagus cuniculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain low activity Mus musculus
-
kidney low activity Mus musculus
-
lung low activity Mus musculus
-
additional information tissue distribution, no activity in testis and liver, overview Mus musculus
-
muscle high activity Mus musculus
-
ovary
-
Mus musculus
-
skeletal muscle
-
Oryctolagus cuniculus
-
spinal cord
-
Mus musculus
-
spleen low activity Mus musculus
-
spleen PAD2 and PAD4 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, calibration using PAD from rabbit skeletal muscle, overview Oryctolagus cuniculus
additional information
-
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Mus musculus
additional information
-
development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Homo sapiens
10.14
-
muscle enzyme Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
Mus musculus N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
Oryctolagus cuniculus N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
Homo sapiens N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
Mus musculus C57BL/6 / 129S N-alpha-benzoyl-L-citrulline ethyl ester + NH3
-
?
protein-L-arginine + H2O
-
Mus musculus protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O
-
Oryctolagus cuniculus protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O
-
Homo sapiens protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Mus musculus protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Oryctolagus cuniculus protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Homo sapiens protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O
-
Mus musculus C57BL/6 / 129S protein-L-citrulline + NH3
-
?
protein-L-arginine + H2O development and evaluation of a highly sensitive antibody-based assay method for PAD activity determination, overview Mus musculus C57BL/6 / 129S protein-L-citrulline + NH3
-
?
SHQESTRGKSKGKAAAAA + H2O
-
Homo sapiens SHQEST-Cit-GKSKGKAAAAA + NH3
-
?

Synonyms

Synonyms Comment Organism
PAD
-
Mus musculus
PAD
-
Oryctolagus cuniculus
PAD
-
Homo sapiens
peptidylarginine deiminase
-
Mus musculus
peptidylarginine deiminase
-
Oryctolagus cuniculus
peptidylarginine deiminase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus
37
-
assay at Oryctolagus cuniculus
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Mus musculus
7.6
-
assay at Oryctolagus cuniculus
7.6
-
assay at Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.5
-
recombinant PAD4, pH 7.6, 37°C Homo sapiens N-alpha-benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine ethyl ester