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Literature summary for 3.5.3.15 extracted from

  • Arita, K.; Shimizu, T.; Hashimoto, H.; Hidaka, Y.; Yamada, M.; Sato, M.
    Structural basis for histone N-terminal recognition by human peptidylarginine deiminase 4 (2006), Proc. Natl. Acad. Sci. USA, 103, 5291-5296.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of GST-tagged PAD4 mutant C645A Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structures of a Ca2+-bound PAD4 mutant C645A in complex with three histone N-terminal peptides, each consisting of 10 amino acid residues that include one target arginine residue for the enzyme (H3/Arg-8, H3/Arg-17, and H4/Arg-3). hanging-drop vapor-diffusion method Homo sapiens
purified recombinant GST-tagged PAD4 mutant C645A in complex with Ca2+ and histone-derived N-terminal peptide substrates, hanging-drop vapor-diffusion method, soaking of Ca2+-free crystals in crystallization buffer containing 5 mM CaCl2 and each histone N-terminal peptide for 8 h at 20°C, X-ray diffraction structure determination and anaylsis at 2.0-2.225 A resolution, molecular replacement Homo sapiens

Protein Variants

Protein Variants Comment Organism
C645A inactive mutant Homo sapiens
C645A site-directed mutagemesis, inactive mutant, substrate-bound structure analysis Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus PAD4 contains nuclear localization signal region in the subdomain 1 Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, binding structure, overview Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
histone H3-L-arginine + H2O Homo sapiens
-
histone H3-L-citrulline + NH3
-
?
protein-L-arginine + H2O Homo sapiens
-
protein-L-citrulline + NH3
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Homo sapiens Q9UM07 PAD4
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged PAD4 mutant C645A by glutathione affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H3-L-arginine + H2O
-
Homo sapiens histone H3-L-citrulline + NH3
-
?
additional information substrate binding structure, sequence specificity, overview Homo sapiens ?
-
?
peptide H3-1-L-arginine + H2O i.e. Ac4KQTARKSTGG13 Homo sapiens peptide H3-1-L-citrulline + NH3
-
?
peptide H3-2-L-arginine + H2O i.e. Ac14KAPRKQLATK23 Homo sapiens peptide H3-2-L-citrulline + NH3
-
?
peptide H4-L-arginine + H2O
-
Homo sapiens peptide H4-L-citrulline + NH3 i.e. Ac1SGRGKGGKGL10 ?
protein-L-arginine + H2O
-
Homo sapiens protein-L-citrulline + NH3
-
?

Synonyms

Synonyms Comment Organism
PAD4
-
Homo sapiens
peptidylarginine deiminase 4
-
Homo sapiens