Cloned (Comment) | Organism |
---|---|
overexpression of GST-tagged PAD4 mutant C645A | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystal structures of a Ca2+-bound PAD4 mutant C645A in complex with three histone N-terminal peptides, each consisting of 10 amino acid residues that include one target arginine residue for the enzyme (H3/Arg-8, H3/Arg-17, and H4/Arg-3). hanging-drop vapor-diffusion method | Homo sapiens |
purified recombinant GST-tagged PAD4 mutant C645A in complex with Ca2+ and histone-derived N-terminal peptide substrates, hanging-drop vapor-diffusion method, soaking of Ca2+-free crystals in crystallization buffer containing 5 mM CaCl2 and each histone N-terminal peptide for 8 h at 20°C, X-ray diffraction structure determination and anaylsis at 2.0-2.225 A resolution, molecular replacement | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C645A | inactive mutant | Homo sapiens |
C645A | site-directed mutagemesis, inactive mutant, substrate-bound structure analysis | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | PAD4 contains nuclear localization signal region in the subdomain 1 | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, binding structure, overview | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3-L-arginine + H2O | Homo sapiens | - |
histone H3-L-citrulline + NH3 | - |
? | |
protein-L-arginine + H2O | Homo sapiens | - |
protein-L-citrulline + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Homo sapiens | Q9UM07 | PAD4 | - |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged PAD4 mutant C645A by glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
histone H3-L-arginine + H2O | - |
Homo sapiens | histone H3-L-citrulline + NH3 | - |
? | |
additional information | substrate binding structure, sequence specificity, overview | Homo sapiens | ? | - |
? | |
peptide H3-1-L-arginine + H2O | i.e. Ac4KQTARKSTGG13 | Homo sapiens | peptide H3-1-L-citrulline + NH3 | - |
? | |
peptide H3-2-L-arginine + H2O | i.e. Ac14KAPRKQLATK23 | Homo sapiens | peptide H3-2-L-citrulline + NH3 | - |
? | |
peptide H4-L-arginine + H2O | - |
Homo sapiens | peptide H4-L-citrulline + NH3 | i.e. Ac1SGRGKGGKGL10 | ? | |
protein-L-arginine + H2O | - |
Homo sapiens | protein-L-citrulline + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAD4 | - |
Homo sapiens |
peptidylarginine deiminase 4 | - |
Homo sapiens |