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Literature summary for 3.5.3.15 extracted from

  • Mechin, M.C.; Enji, M.; Nachat, R.; Chavanas, S.; Charveron, M.; Ishida-Yamamoto, A.; Serre, G.; Takahara, H.; Simon, M.
    The peptidylarginine deiminases expressed in human epidermis differ in their substrate specificities and subcellular locations (2005), Cell. Mol. Life Sci., 62, 1984-1995.
    View publication on PubMed
Search for more literature for 3.5.3.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.35
-
 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
0.38
-
 benzoyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
0.5
-
 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 2 Homo sapiens
0.91
-
 acetyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
1.19
-
 acetyl-L-Arg methyl ester pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
1.49
-
 benzoyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 2 Homo sapiens
3.73
-
 acetyl-L-Arg methyl ester pH 7.6, 55°C, peptidylarginine deiminase 2 Homo sapiens
7.5
-
 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 3 Homo sapiens
33.6
-
 benzoyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 3 Homo sapiens
66.3
-
 acetyl-L-Arg methyl ester pH 7.6, 55°C, peptidylarginine deiminase 3 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM required for maximal deimination of filaggrin, peptidylarginine deiminase 1. Maximal deamination of benzoyl-L-Arg ethyl ester at 4 mM or above Homo sapiens
Ca2+ 1.5 mM required for maximal deimination of filaggrin, peptidylarginine deiminase 3, maximal deimination of benzoyl-L-Arg ethyl ester at 4 mM or above Homo sapiens
Ca2+ 2.5 mM required for maximal deamination of filaggrin, peptidylarginine deiminase 2. Maximal deimination of benzoyl-L-Arg ethyl ester at 4 mM or above Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 peptidylarginine deiminase 2
-
Homo sapiens Q9ULC6 peptidylarginine deiminase 1
-
Homo sapiens Q9ULW8 peptidylarginine deiminase 3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant peptidylarginine deiminase 1 Homo sapiens
recombinant peptidylarginine deiminase 2 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
epidermis peptidylarginine deiminase 1 Homo sapiens
-
epidermis peptidylarginine deiminase 2 Homo sapiens
-
epidermis peptidylarginine deiminase 3 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.434
-
 substrate: benzoyl-L-Arg ethyl ester, 55°C, peptidylarginine deiminase 2 Homo sapiens
0.583
-
 substrate: benzoyl-L-Arg ethyl ester, 55°C, peptidylarginine deiminase 1 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-L-Arg + H2O peptidylarginine deiminase 1 Homo sapiens acetyl-L-citrulline + NH3
-
 ?
acetyl-L-Arg methyl ester + H2O peptidylarginine deiminase 1 Homo sapiens acetyl-L-citrulline methyl ester + NH3
-
 ?
acetyl-L-Arg methyl ester + H2O peptidylarginine deiminase 2 Homo sapiens acetyl-L-citrulline methyl ester + NH3
-
 ?
acetyl-L-Arg methyl ester + H2O peptidylarginine deiminase 3 Homo sapiens acetyl-L-citrulline methyl ester + NH3
-
 ?
benzoyl-L-Arg + H2O peptidylarginine deiminase 1 Homo sapiens benzoyl-L-citrulline + NH3
-
 ?
benzoyl-L-Arg + H2O peptidylarginine deiminase 2 Homo sapiens benzoyl-L-citrulline + NH3
-
 ?
benzoyl-L-Arg + H2O peptidylarginine deiminase 3 Homo sapiens benzoyl-L-citrulline + NH3
-
 ?
benzoyl-L-Arg ethyl ester + H2O peptidylarginine deiminase 1 Homo sapiens benzoyl-L-citrulline ethyl ester + NH3
-
 ?
benzoyl-L-Arg ethyl ester + H2O peptidylarginine deiminase 2 Homo sapiens benzoyl-L-citrulline ethyl ester + NH3
-
 ?
benzoyl-L-Arg ethyl ester + H2O peptidylarginine deiminase 3 Homo sapiens benzoyl-L-citrulline ethyl ester + NH3
-
 ?
filaggrin L-arginine + H2O peptidylarginine deiminase 1 Homo sapiens filaggrin L-citrulline + NH3
-
 ?
filaggrin L-arginine + H2O peptidylarginine deiminase 2 Homo sapiens filaggrin L-citrulline + NH3
-
 ?
filaggrin L-arginine + H2O peptidylarginine deiminase 3 Homo sapiens filaggrin L-citrulline + NH3
-
 ?
keratin L-arginine + H2O peptidylarginine deiminase 1 Homo sapiens keratin L-citrulline + NH3
-
 ?
keratin L-arginine + H2O peptidylarginine deiminase 2 Homo sapiens keratin L-citrulline + NH3
-
 ?
keratin L-arginine + H2O peptidylarginine deiminase 3 Homo sapiens keratin L-citrulline + NH3
-
 ?

Synonyms

Synonyms Comment Organism
PAD1
-
 Homo sapiens
PAD2
-
 Homo sapiens
PAD3
-
 Homo sapiens
peptidylarginine deiminase 1
-
 Homo sapiens
peptidylarginine deiminase 2
-
 Homo sapiens
peptidylarginine deiminase 3
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.057 0.65 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
0.057 0.65 acetyl-L-Arg methyl ester pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
0.52
-
 benzoyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
0.55
-
 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 2 Homo sapiens
0.79
-
 benzoyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 3 Homo sapiens
1.73
-
 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 3 Homo sapiens
1.93
-
 acetyl-L-Arg methyl ester pH 7.6, 55°C, peptidylarginine deiminase 3 Homo sapiens
3.15
-
 benzoyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 2 Homo sapiens
6.22
-
 acetyl-L-Arg methyl ester pH 7.6, 55°C, peptidylarginine deiminase 2 Homo sapiens
11.28
-
 acetyl-L-Arg methyl ester pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
11.31
-
 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
13.68
-
 acetyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
14.43
-
 benzoyl-L-Arg pH 7.6, 55°C, peptidylarginine deiminase 1 Homo sapiens
15.24
-
 benzoyl-L-Arg ethyl ester pH 7.6, 55°C, peptidylarginine deiminase 2 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 deamination of fillagrin, peptidylarginine deiminase 3 Homo sapiens
8
-
 deamination of fillagrin, peptidylarginine deiminase 1 Homo sapiens
8
-
 deamination of fillagrin, peptidylarginine deiminase 2 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5.2 8.9 pH 5.2: about 60% of maximal activity, pH 8.9: about 85% of maximal activity, deamination of fillagrin, peptidylarginine deiminase 1 Homo sapiens
5.2 8.9 pH 5.2: about 70% of maximal activity, pH 8.9: about 50% of maximal activity, deimination of fillagrin, peptidylarginine deiminase 3 Homo sapiens
5.2 8.9 pH 5.2: about 70% of maximal activity, pH 8.9: about 75% of maximal activity, deimination of fillagrin, peptidylarginine deiminase 2 Homo sapiens