Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.15 extracted from

  • Kearney, P.L.; Bhatia, M.; Jones, N.G.; Yuan, L.; Glascock, M.C.; Catchings, K.L.; Yamada, M.; Thompson, P.R.
    Kinetic characterization of protein arginine deiminase 4: a transcriptional corepressor implicated in the onset and progression of rheumatoid arthritis (2005), Biochemistry, 44, 10570-10582.
    View publication on PubMed
Search for more literature for 3.5.3.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 benzoyl-L-arginine amide 37°C Homo sapiens
0.41
-
 benzoyl-L-arginine 37°C Homo sapiens
1.36
-
 benzoyl-L-arginine ethyl ester 37°C Homo sapiens
1.66
-
 benzoyl-L-arginine methyl ester 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ PAD4 activity is highly specific for calcium. Calcium activation of PAD4 catalysis exhibits positive cooperativity. ca2+ causes conformational change Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9UM07 recombinant
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, 10% 10 mM Tris-HCl, pH 8.0, 1 mM EDTA, 500 mM NaCl, 1 mM DTT and 10% glycerol, stable for several months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-L-arginine + H2O
-
 Homo sapiens benzoyl-L-citrulline + NH3
-
 ?
benzoyl-L-arginine amide + H2O
-
 Homo sapiens benzoyl-L-citrulline amide + NH3
-
 ?
benzoyl-L-arginine ethyl ester + H2O
-
 Homo sapiens benzoyl-L-citrulline ethyl ester + NH3
-
 ?
benzoyl-L-arginine methyl ester + H2O
-
 Homo sapiens benzoyl-L-citrulline methyl ester + NH3
-
 ?
additional information methylated Arg residues are quite poor substrates for full-length PAD4 in vitro, regardless of whether this residue is in the context of a small molecule or a peptide substrate Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PAD4
-
 Homo sapiens
protein arginine deiminase 4
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.76
-
 benzoyl-L-arginine amide 37°C Homo sapiens
3 6 benzoyl-L-arginine amide 37°C Homo sapiens
3.35
-
 benzoyl-L-arginine 37°C Homo sapiens
5.57
-
 benzoyl-L-arginine methyl ester 37°C Homo sapiens
5.94
-
 benzoyl-L-arginine ethyl ester 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8 pH 6.5: about 50% of maximal activity, pH 8.0: about 70% of maximal activity Homo sapiens