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Literature summary for 3.5.3.1 extracted from

  • El-Sayed, A.S.; Shindia, A.A.; Diab, A.A.; Rady, A.M.
    Purification and immobilization of L-arginase from thermotolerant Penicillium chrysogenum KJ185377.1; with unique kinetic properties as thermostable anticancer enzyme (2014), Arch. Pharm. Res., 0, doi.org/10.1007/s12272-014-0498-y.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
hydroxylamine
-
Penicillium chrysogenum
iodoacetamide
-
Penicillium chrysogenum
propargylglycine
-
Penicillium chrysogenum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8
-
L-arginine free and chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
15.2
-
L-arginine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
22.1
-
L-phenylalanine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
32.8
-
L-phenylalanine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
57.5
-
L-phenylalanine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Penicillium chrysogenum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
x * 37000, SDS-PAGE Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-arginine + H2O Penicillium chrysogenum
-
L-ornithine + urea
-
?
L-arginine + H2O Penicillium chrysogenum KJ185377.1
-
L-ornithine + urea
-
?

Organism

Organism UniProt Comment Textmining
Penicillium chrysogenum
-
-
-
Penicillium chrysogenum KJ185377.1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE-Sepharose column chromatography and gel filtration Penicillium chrysogenum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.44
-
crude enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
24.45
-
after 10.02fold purification, at pH 7.0 and 40°C Penicillium chrysogenum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
Penicillium chrysogenum L-ornithine + urea
-
?
L-arginine + H2O
-
Penicillium chrysogenum KJ185377.1 L-ornithine + urea
-
?
L-phenylalanine + H2O
-
Penicillium chrysogenum 2-oxo-3-phenylpropanoate + NH3
-
?
L-phenylalanine + H2O
-
Penicillium chrysogenum KJ185377.1 2-oxo-3-phenylpropanoate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE Penicillium chrysogenum

Synonyms

Synonyms Comment Organism
ARG
-
Penicillium chrysogenum
L-arginine amidinohydrolase
-
Penicillium chrysogenum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Penicillium chrysogenum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.18
-
L-arginine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.25
-
L-arginine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.25
-
L-phenylalanine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.27
-
L-arginine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.46
-
L-phenylalanine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.94
-
L-phenylalanine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.8 7.9
-
Penicillium chrysogenum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0305
-
propargylglycine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0362
-
iodoacetamide chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0442
-
hydroxylamine chitosan-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0618
-
iodoacetamide free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0626
-
propargylglycine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0656
-
hydroxylamine PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0658
-
iodoacetamide PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0808
-
hydroxylamine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum
0.0857
-
propargylglycine free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0618
-
free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum iodoacetamide
0.0626
-
PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum propargylglycine
0.0656
-
PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum hydroxylamine
0.0658
-
PEG-immobilized enzyme, at pH 7.0 and 40°C Penicillium chrysogenum iodoacetamide
0.0808
-
free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum hydroxylamine
0.0857
-
free enzyme, at pH 7.0 and 40°C Penicillium chrysogenum propargylglycine