Cloned (Comment) | Organism |
---|---|
expression of apo-enzyme in a Mn2+- and Co2+-free minimal medium | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Helicobacter pylori | |
dithiothreitol | - |
Helicobacter pylori | |
Nomega-hydroxy-nor-arginine | - |
Helicobacter pylori | |
S-(2-boronoethyl)-L-cysteine | - |
Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | metal preference in decreasing order: Co2+, Ni2+, Mn2+. Heat-activation in presence of metal ion is essential for activation of apo-enzyme | Helicobacter pylori | |
Mn2+ | metal preference in decreasing order: Co2+, Ni2+, Mn2+. Heat-activation in presence of metal ion is essential for activation of apo-enzyme | Helicobacter pylori | |
Ni2+ | metal preference in decreasing order: Co2+, Ni2+, Mn2+. Heat-activation in presence of metal ion is essential for activation of apo-enzyme | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | O25949 | - |
- |
Subunits | Comment | Organism |
---|---|---|
monomer | apo-form of enzyme, gel fitlration | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
RocF | - |
Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
apo-form of enzyme | Helicobacter pylori |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | - |
no residual activity | Helicobacter pylori |
9 | - |
50% of maximum activity | Helicobacter pylori |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.01 | - |
pH 7.5, 37°C | Helicobacter pylori | dithiothreitol | |
0.03 | - |
pH 7.5, 37°C | Helicobacter pylori | 2-mercaptoethanol | |
0.5 | - |
pH 7.5, 37°C | Helicobacter pylori | Nomega-hydroxy-nor-arginine | |
1 | - |
pH 7.5, 37°C | Helicobacter pylori | S-(2-boronoethyl)-L-cysteine |