Protein Variants | Comment | Organism |
---|---|---|
A92S | mutation of A92 to its analogous residues in other arginases individually enhances the catalytic activity | Helicobacter pylori |
D116A | no detectable activity | Helicobacter pylori |
E90A | residue E90 is important for catalytic activity and plays a crucial role in retaining the metal ion at the active site | Helicobacter pylori |
E90A/H118A | no detectable activity | Helicobacter pylori |
H91A | residue E90 is important for catalytic activity and plays a crucial role in retaining the metal ion at the active site | Helicobacter pylori |
S88G | mutation of Ser88 to its analogous residues in other arginases individually enhances the catalytic activity | Helicobacter pylori |
S88G/A92S | mutation of S88 and A92 to its analogous residues in other arginases individually enhances the catalytic activity | Helicobacter pylori |
S89G | 1.5fold decrease in catalytic activity | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.1 | - |
L-arginine | mutant S89G, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.1 | Helicobacter pylori | |
4.4 | - |
L-arginine | mutant H91A, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.3 | Helicobacter pylori | |
5.2 | - |
L-arginine | mutant A92S, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 1.6 | Helicobacter pylori | |
5.9 | - |
L-arginine | mutant A92S, Co2+-activated, pH 5.0, 37°C, Hill coefficient 1.9 | Helicobacter pylori | |
5.9 | - |
L-arginine | mutant S89G, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.2 | Helicobacter pylori | |
6.3 | - |
L-arginine | mutant S88G, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.0 | Helicobacter pylori | |
6.4 | - |
L-arginine | mutant E90A, Co2+-activated, pH 5.0, 37°C, Hill coefficient 1.7 | Helicobacter pylori | |
6.7 | - |
L-arginine | mutant S88G/A92S, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.0 | Helicobacter pylori | |
6.7 | - |
L-arginine | wild-type, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 1.6 | Helicobacter pylori | |
8.4 | - |
L-arginine | wild-type, Co2+-activated, pH 5.0, 37°C, Hill coefficient 2.1 | Helicobacter pylori | |
8.7 | - |
L-arginine | mutant S88G, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.4 | Helicobacter pylori | |
9.2 | - |
L-arginine | mutant S88G/A92S, Mn2+-activated, pH 5.0, 37°C, Hill coefficient 2.2 | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Helicobacter pylori | L-ornithine + urea | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
69 | - |
melting temperature, apo-form of wild-type | Helicobacter pylori |
71 | - |
melting temperature, holo-form of wild-type | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
L-arginine | mutant E90A, Co2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.013 | - |
L-arginine | mutant H91A, Co2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.04 | - |
L-arginine | mutant S89G, Mn2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.067 | - |
L-arginine | wild-type, Mn2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.12 | - |
L-arginine | mutant S89G, Co2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.27 | - |
L-arginine | mutant S88G, Mn2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.28 | - |
L-arginine | mutant S88G/A92S, Mn2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.28 | - |
L-arginine | wild-type, Co2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.84 | - |
L-arginine | mutant A92S, Mn2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
0.92 | - |
L-arginine | mutant A92S, Co2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
1.01 | - |
L-arginine | mutant S88G, Co2+-activated, pH 5.0, 37°C | Helicobacter pylori | |
1.37 | - |
L-arginine | mutant S88G/A92S, Co2+-activated, pH 5.0, 37°C | Helicobacter pylori |