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Literature summary for 3.5.3.1 extracted from

  • Chen, H.; McCaig, B.C.; Melotto, M.; He, S.Y.; Howe, G.A.
    Regulation of plant arginase by wounding, jasmonate, and the phytotoxin coronatine (2004), J. Biol. Chem., 279, 45998-46007.
    View publication on PubMed
Search for more literature for 3.5.3.1

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptopropionate 5 mM, isoform LeARG1, 38% residual activity, LeARG2, 38% residual activity; 5 mM, isoform LeARG1, 38% residual activity, LeARG2, 38% residual activity Solanum lycopersicum
3-Mercaptopropionate 5 mM, isoform LeARG1, 24% residual activity, LeARG2, 26% residual activity; 5 mM, isoform LeARG1, 24% residual activity, LeARG2, 26% residual activity Solanum lycopersicum
Mercaptoacetate 5 mM, isoform LeARG1, 28% residual activity, LeARG2, 26% residual activity; 5 mM, isoform LeARG1, 28% residual activity, LeARG2, 26% residual activity Solanum lycopersicum
Nomega-hydroxy-L-arginine 0.2 mM, isoform LeARG1, 7% residual activity, LeARG2, 5% residual activity; 0.2 mM, isoform LeARG1, 7% residual activity, LeARG2, 5% residual activity Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, SDS-PAGE and calculated Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum Q5UNS1 isoform LeARG2; isoform LeARG2
-
Solanum lycopersicum Q5UNS2 isoform LeARG1; enzyme isoform LeARG1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf induction of isoform LeARG2 upon wounding and treatment with jasmonic acid Solanum lycopersicum
-
additional information highest levels of enzyme activity in reproductive tissues Solanum lycopersicum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O isoform LeARG1, 0.5% of the activity with L-arginine, LeARG2, 0.6% of the activity with L-arginine Solanum lycopersicum 1,4-diaminobutane + urea
-
 ?
D-arginine + H2O isoform LeARG1, 2.2% of the activity with L-arginine, LeARG2, 3.6% of the activity with L-arginine Solanum lycopersicum D-ornithine + urea
-
 ?
L-arginine + H2O
-
 Solanum lycopersicum L-ornithine + urea
-
 ?
L-homoarginine + H2O isoform LeARG1, 14% of the activity with L-arginine, LeARG2, 13% of the activity with L-arginine Solanum lycopersicum L-2,6-diaminohexanoate + urea
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, SDS-PAGE and calculated Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Solanum lycopersicum

pH Range

pH Minimum pH Maximum Comment Organism
7 12 no activity below or above this range Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.014
-
 Nomega-hydroxy-L-arginine pH 9.6, 37°C Solanum lycopersicum