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Literature summary for 3.5.3.1 extracted from

  • Dabir, S.; Dabir, P.; Somvanshi, B.
    Purification, properties and alternate substrate specificities of arginase from two different sources: Vigna catjang cotyledon and buffalo liver (2005), Int. J. Biol. Sci., 1, 114-122.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
L-arginine pH 9.2, 37°C Bos taurus
42
-
L-arginine pH 10.0, 37°C Vigna catjang

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 52% of the activity with Mn2+ Bos taurus
Ca2+ 35% of the activity with Mn2+ Vigna catjang
Co2+ 45% of the activity with Mn2+ Bos taurus
Co2+ 66% of the activity with Mn2+ Vigna catjang
Fe2+ 52% of the activity with Mn2+ Vigna catjang
Mg2+ 61% of the activity with Mn2+ Vigna catjang
Mg2+ 93% of the activity with Mn2+ Bos taurus
Mn2+ maximal activity at 0.6 mM Vigna catjang
Mn2+ maximal activity at 2.0 mM Bos taurus
additional information no activity with Fe2+, Zn2+ Bos taurus
Ni2+ 51% of the activity with Mn2+ Bos taurus
Ni2+ 55% of the activity with Mn2+ Vigna catjang
Zn2+ 38% of the activity with Mn2+ Vigna catjang

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
x * 43000, SDS-PAGE Bos taurus
52000
-
4 * 52000, SDS-PAGE Vigna catjang
120000
-
gel filtration Bos taurus
210000
-
gel filtration Vigna catjang

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Vigna catjang
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus
-
Vigna catjang

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Vigna catjang
-
liver
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2045
-
pH 10.0, 37°C Vigna catjang
91300
-
pH 9.2, 37°C Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agmatine + H2O 16% of the activity with L-arginine Vigna catjang 1,4-diaminobutane + urea
-
?
canavanine + H2O 12.7% of the activity with L-arginine Vigna catjang urea + NH2OCH2CH2CH(NH2)COOH
-
?
canavanine + H2O 9.6% of the activity with L-arginine Bos taurus urea + NH2OCH2CH2CH(NH2)COOH
-
?
L-arginine + H2O
-
Bos taurus L-ornithine + urea
-
?
L-arginine + H2O
-
Vigna catjang L-ornithine + urea
-
?
additional information no substrate: agmatine, D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin Bos taurus ?
-
?
additional information no substrate: D-arginine, L-homoarginine, L-argininic acid, gamma-guanidinobutyric acid, beta-guanidinopropionic acid, streptomycin Vigna catjang ?
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Bos taurus
tetramer 4 * 52000, SDS-PAGE Vigna catjang

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Vigna catjang
42
-
-
Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
stable Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
10
-
-
Bos taurus
10
-
-
Vigna catjang