Protein Variants | Comment | Organism |
---|---|---|
H120N | 53% of wild-type activity, no alteration in KM-value | Homo sapiens |
H145N | 95% of wild-type activity, no alteration in KM-value | Homo sapiens |
N149D | almost complete loss of activity with L-arginine, gain of activity with agmatine | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
L-arginine | 37°C, pH 9.0, wild-type | Homo sapiens | |
1.5 | - |
L-arginine | 37°C, pH 9.0, mutant H145N | Homo sapiens | |
1.6 | - |
L-arginine | 37°C, pH 9.0, mutant H120N | Homo sapiens | |
2.5 | - |
agmatine | 37°C, pH 9.0, mutant N149D | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activation. after dialysis with EDTA, wild-type and mutant H145N contain 1.1 and 1.3 Mn2+ per subunit, resp., and are hlaf-.active. Mutant H120N contains less than 0.1 Mn2+ per subunit and is inactive | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform arginase II | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
agmatine + H2O | for wild-type, no substrate. Hydrolysis by mutant N149D | Homo sapiens | 1,4-diaminobutane + urea | - |
? | |
L-arginine + H2O | - |
Homo sapiens | L-ornithine + urea | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
131 | - |
L-arginine | 37°C, pH 9.0, mutant H120N | Homo sapiens | |
238 | - |
L-arginine | 37°C, pH 9.0, mutant H145N | Homo sapiens | |
249 | - |
L-arginine | 37°C, pH 9.0, wild-type | Homo sapiens |