Application | Comment | Organism |
---|---|---|
medicine | enzyme is a target for inhibitors used in therapeutic treatment of smooth muscle disorders, such as erectile dysfunction | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and truncated mutant enzymes in Escherichia coli BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
7 mg/ml purified fully active, untruncated enzyme complexed with 5 mM inhibitor S-(2-boronoethyl)-L-cysteine in 50 mM bicine, pH 8.5, 0.003 ml mixed with 0.1 ml MnCl2 and 0.003 ml precipitant solution containing 0.1 M Tris-HCl, pH 8.3-8.5, 3 M ammonium sulfate, equilibration over 1 ml reservoir solution containing 0.1 M Tris-HCl, pH 8.3-8.5, 3 M ammonium sulfate, and 20% v/v glycerol, hanging drop vapour diffusion method at 4°C, X-ray diffraction structure determination and analysis at 2.7 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of the truncated mutant DELTAM1-V23/DELTAH331-I354 showing reduced acivity compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2(S)-amino-6-boronohexanoic acid | transition state analogue inhibitor | Homo sapiens | |
2(S)-amino-6-boronohexanoic acid | transition state analogue inhibitor, effect on female and male hemodynamics, overview | Oryctolagus cuniculus | |
S-(2-boronoethyl)-L-cysteine | transition state analogue inhibitor | Homo sapiens | |
S-(2-boronoethyl)-L-cysteine | transition state analogue inhibitor | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
L-arginine | wild-type enzyme, pH 9.5 | Homo sapiens | |
2.4 | - |
L-arginine | truncated mutant enzyme, pH 9.5 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | manganese-metalloenzyme, binuclear cluster, binding structure | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | Oryctolagus cuniculus | - |
L-ornithine + urea | - |
ir | |
L-arginine + H2O | Homo sapiens | enzyme is involved in regulating L-arginine bioavailability to NO synthase in human penile corpus cavernosum smooth muscle, and in clitoral corpus cavernosum and vagina, enzyme plays a role in both female and male sexual arousal | L-ornithine + urea | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme II | - |
Oryctolagus cuniculus | - |
white New Zealand rabbits, isozyme II | - |
Purification (Comment) | Organism |
---|---|
partially from vaginal tissue | Oryctolagus cuniculus |
recombinant wild-type and truncated mutant enzymes from Escherichia coli BL21(DE3) | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-arginine + H2O = L-ornithine + urea | hydrolysis via a metall-activated hydroxide mechanism, substrate binding structure | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
corpus cavernosum clitoridis | - |
Homo sapiens | - |
corpus cavernosum clitoridis | - |
Oryctolagus cuniculus | - |
corpus cavernosum penis | smooth muscle | Homo sapiens | - |
corpus cavernosum penis | smooth muscle | Oryctolagus cuniculus | - |
additional information | extrahepatic | Homo sapiens | - |
additional information | extrahepatic | Oryctolagus cuniculus | - |
smooth muscle | of penile corpus cavernosum | Homo sapiens | - |
smooth muscle | of penile corpus cavernosum | Oryctolagus cuniculus | - |
vagina | - |
Homo sapiens | - |
vagina | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + H2O | - |
Homo sapiens | L-ornithine + urea | - |
ir | |
L-arginine + H2O | - |
Oryctolagus cuniculus | L-ornithine + urea | - |
ir | |
L-arginine + H2O | enzyme is involved in regulating L-arginine bioavailability to NO synthase in human penile corpus cavernosum smooth muscle, and in clitoral corpus cavernosum and vagina, enzyme plays a role in both female and male sexual arousal | Homo sapiens | L-ornithine + urea | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | monomer-monomer-interactions are stabilized by a arginine-rich network of intersubunit salt bridges, sulfate counterions, and hydrogen bonds more extensive than that observed in isozyme I | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
arginase II | - |
Homo sapiens |
arginase II | - |
Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
203 | - |
L-arginine | truncated mutant enzyme, pH 9.5 | Homo sapiens | |
231 | - |
L-arginine | wild-type enzyme, pH 9.5 | Homo sapiens |