Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.3.1 extracted from

  • Moreno-Vivian, C.; Soler, G.; Castillo, F.
    Arginine catabolism in the phototrophic bacterium Rhodobacter capsulatus E1F1. Purification and properties of arginase (1992), J. Biochem., 204, 531-537.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
putrescine activating Rhodobacter capsulatus

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol
-
Rhodobacter capsulatus
Borate
-
Rhodobacter capsulatus
Cu2+
-
Rhodobacter capsulatus
ethanol
-
Rhodobacter capsulatus
gamma-guanidinobutyrate
-
Rhodobacter capsulatus
L-ornithine
-
Rhodobacter capsulatus
p-hydroxymercuribenzoate
-
Rhodobacter capsulatus
Zn2+
-
Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
16
-
L-Arg
-
Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activating Rhodobacter capsulatus
Fe2+ slight activation Rhodobacter capsulatus
Mn2+ activating Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
4 * 31000, SDS-PAGE Rhodobacter capsulatus
117000
-
gel filtration and sucrose density gradient ultracentrifugation Rhodobacter capsulatus
126000
-
non-denaturing PAGE Rhodobacter capsulatus
138000
-
gel filtration Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
E1F1
-
Rhodobacter capsulatus E1F1
-
E1F1
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
183
-
-
Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + H2O
-
Rhodobacter capsulatus L-ornithine + urea
-
?
L-arginine + H2O
-
Rhodobacter capsulatus E1F1 L-ornithine + urea
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 31000, SDS-PAGE Rhodobacter capsulatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
-20
-
no loss of activity for several months Rhodobacter capsulatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5 9.5
-
Rhodobacter capsulatus