Cloned (Comment) | Organism |
---|---|
gene blaNDM-1, genotyping, recombinant N-terminally His6-tagged enzyme expression in Escherichia coli strain BL21(DE3) | Klebsiella pneumoniae |
gene blaNDM-10, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Klebsiella pneumoniae |
gene blaNDM-12, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
gene blaNDM-2, genotyping, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Acinetobacter baumannii |
gene blaNDM-3, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
gene blaNDM-4, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
gene blaNDM-5, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
gene blaNDM-6, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
gene blaNDM-7, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
gene blaNDM-8, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Escherichia coli |
gene blaNDM-9, genotyping, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
A233V | naturally occuring genetic variant | Escherichia coli |
D130G/M154L | naturally occuring genetic variation | Escherichia coli |
D130N/M154L | naturally occuring genetic variation | Escherichia coli |
D95N | naturally occuring genetic variation | Escherichia coli |
E152K | naturally occuring genetic variation | Klebsiella pneumoniae |
G69S/A74T/G200R | naturally occuring genetic variation | Klebsiella pneumoniae |
M154L | naturally occuring genetic variation | Escherichia coli |
M154L/G222D | naturally occuring genetic variation | Escherichia coli |
P28A | naturally occuring genetic variation, the P28A substitution occurs in the predicted N-terminal periplasmic signal peptide | Acinetobacter baumannii |
V88L/M154L | naturally occuring genetic variation | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nitrocefin | substrate inhibition; substrate inhibition; substrate inhibition; substrate inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.005 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.005 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.006 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.008 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.01 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.011 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.011 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.013 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.015 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.016 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.019 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.02 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.023 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.027 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.029 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.043 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.045 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.049 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.05 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.054 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.056 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.057 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.058 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.062 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.063 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.064 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.078 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.079 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.082 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.083 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.086 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.099 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.1 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.107 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.11 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.115 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.119 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.119 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
0.119 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.125 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.126 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.133 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.144 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.148 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.151 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.156 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.207 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.229 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.258 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.305 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.455 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Klebsiella pneumoniae | - |
- |
periplasm | - |
Acinetobacter baumannii | - |
- |
periplasm | - |
Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | a zinc metalloenzyme | Klebsiella pneumoniae | |
Zn2+ | a zinc metalloenzyme | Acinetobacter baumannii | |
Zn2+ | a zinc metalloenzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter baumannii | F2YZ26 | gene blaNDM-2 | - |
Escherichia coli | A0A024FRL9 | gene blaNDM-12 | - |
Escherichia coli | A0A0F6N6D4 | gene blaNDM-5 | - |
Escherichia coli | H6WET3 | gene blaNDM-6 | - |
Escherichia coli | H6WZS9 | gene blaNDM-4 | - |
Escherichia coli | I3VKD5 | gene blaNDM-3 | - |
Escherichia coli | J7I0S9 | gene blaNDM-7 | - |
Escherichia coli | M1VE66 | gene blaNDM-8 | - |
Klebsiella pneumoniae | C7C422 | gene blaNDM-1 | - |
Klebsiella pneumoniae | S5ZIP8 | gene blaNDM-10 | - |
Klebsiella pneumoniae | T2A6Y2 | gene blaNDM-9 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the N-terminal His6-tag using recombinant human Rhinovirus 3C protease, and further purification of the untagged protein by affinity chromatography, followed by ultrafiltration | Klebsiella pneumoniae |
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the N-terminal His6-tag using recombinant human Rhinovirus 3C protease, and further purification of the untagged protein by affinity chromatography, followed by ultrafiltration | Acinetobacter baumannii |
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the N-terminal His6-tag using recombinant human Rhinovirus 3C protease, and further purification of the untagged protein by affinity chromatography, followed by ultrafiltration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Klebsiella pneumoniae | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Acinetobacter baumannii | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Escherichia coli | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefalotin + H2O | - |
Klebsiella pneumoniae | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefalotin + H2O | - |
Escherichia coli | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | low activity | Klebsiella pneumoniae | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefoxitin + H2O | low activity | Escherichia coli | (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[2-(thiophen-2-yl)acetamido]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | low activity | Klebsiella pneumoniae | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
ceftazidime + H2O | low activity | Escherichia coli | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino](carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
doripenem + H2O | - |
Klebsiella pneumoniae | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-([(3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
doripenem + H2O | - |
Escherichia coli | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-4-methyl-3-([(3S,5S)-5-[(sulfamoylamino)methyl]pyrrolidin-3-yl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
imipenem + H2O | - |
Klebsiella pneumoniae | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([2-[(iminomethyl)amino]ethyl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
imipenem + H2O | - |
Escherichia coli | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-([2-[(iminomethyl)amino]ethyl]sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
meropenem + H2O | - |
Klebsiella pneumoniae | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
meropenem + H2O | - |
Escherichia coli | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Klebsiella pneumoniae | (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Escherichia coli | (2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MBL | - |
Klebsiella pneumoniae |
MBL | - |
Acinetobacter baumannii |
MBL | - |
Escherichia coli |
NDM | - |
Klebsiella pneumoniae |
NDM | - |
Acinetobacter baumannii |
NDM | - |
Escherichia coli |
NDM-1 | - |
Klebsiella pneumoniae |
NDM-10 | - |
Klebsiella pneumoniae |
NDM-12 | - |
Escherichia coli |
NDM-2 | - |
Acinetobacter baumannii |
NDM-3 | - |
Escherichia coli |
NDM-4 | - |
Escherichia coli |
NDM-5 | - |
Escherichia coli |
NDM-6 | - |
Escherichia coli |
NDM-7 | - |
Escherichia coli |
NDM-8 | - |
Escherichia coli |
NDM-9 | - |
Klebsiella pneumoniae |
New Delhi MBL | - |
Klebsiella pneumoniae |
New Delhi MBL | - |
Acinetobacter baumannii |
New Delhi MBL | - |
Escherichia coli |
New Delhi metallo-beta-lactamase | - |
Klebsiella pneumoniae |
New Delhi metallo-beta-lactamase | - |
Acinetobacter baumannii |
New Delhi metallo-beta-lactamase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Klebsiella pneumoniae |
25 | - |
assay at | Acinetobacter baumannii |
25 | - |
assay at | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55.1 | - |
melting temperature of the purified NDM-1 variant | Escherichia coli |
59.5 | - |
melting temperature of the purified NDM-1 variant | Klebsiella pneumoniae |
61.4 | - |
melting temperature of the purified NDM-1 variant | Escherichia coli |
63.4 | - |
melting temperature of the purified NDM-1 variant | Escherichia coli |
64.6 | - |
melting temperature of the purified NDM-1 variant | Escherichia coli |
72.1 | - |
melting temperature of the purified NDM-1 variant | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
3 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
5 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
6 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
9 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
10 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
14 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
15 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
15 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
16 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
18 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
18 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
23 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
24 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
25 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
29 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
30 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
33 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
38 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
44 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
45 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
49 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
53 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
54 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
63 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
65 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
75 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
75 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
127 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
142 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
170 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
194 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
226 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
238 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
252 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
267 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
273 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
301 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
332 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
348 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
355 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
362 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
408 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
413 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
447 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
477 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
583 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
600 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
724 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
743 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
757 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
900 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1032 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1375 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Klebsiella pneumoniae |
7.2 | - |
assay at | Acinetobacter baumannii |
7.2 | - |
assay at | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.079 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.102 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.139 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
0.146 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
additional information | different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution. Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview | Klebsiella pneumoniae |
additional information | different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution. Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview | Acinetobacter baumannii |
additional information | different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution. Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview | Escherichia coli |
additional information | different genotypes of the New Delhi metallo beta-lactamase are identified: the substitutions do not affect the overall folds of the enzyme variants, within limits of detection. Differences in thermal stabilities are observed. kcat/KM values are similar for carbapenem and penicillin substrates for NDM variants, but differences in kinetics are observed for cephalosporin substrates. Apparent substrate inhibition is observed with nitrocefin for variants containing the M154L substitution. In all cases, cefoxitin and ceftazidime are poorly hydrolysed. Clinically observed substitutions can make substantial differences in thermodynamic stability, suggesting that this may be a factor in metallo beta-lactamase evolution.Comparative analysis of the beta-lactam susceptibility of the NDM variants in Escherichia coli, overview | Escherichia coli |
physiological function | New Delhi MBL (NDM)-1 is a clinically significant metallo beta-lactamase | Klebsiella pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
130 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
140 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
150 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
170 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
180 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
180 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
200 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
210 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
220 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
230 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
230 | - |
cefoxitin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
260 | - |
ceftazidime | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1200 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1500 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1600 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1700 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1900 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
1900 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
2000 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2200 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2300 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2300 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2600 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2600 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2600 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
2800 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2900 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
2900 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
3000 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
3100 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4100 | - |
ampicillin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
4100 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4100 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4100 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4200 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4300 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4500 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4600 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
4900 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
5200 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
5400 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
5600 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
5800 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
5900 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
6000 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
6100 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
6100 | - |
cefalotin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
6300 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
7400 | - |
meropenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
7500 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
7600 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Klebsiella pneumoniae | |
8600 | - |
doripenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
9200 | - |
Imipenem | pH 7.2, 25°C, recombinant enzyme | Escherichia coli | |
10900 | - |
nitrocefin | pH 7.2, 25°C, recombinant enzyme | Escherichia coli |