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Literature summary for 3.5.2.6 extracted from

  • Hwang, J.; Cho, K.H.; Song, H.; Yi, H.; Kim, H.S.
    Deletion mutations conferring substrate spectrum extension in the class A beta-lactamase (2014), Antimicrob. Agents Chemother., 58, 6265-6269.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Protein Variants

Protein Variants Comment Organism
A172E random mutagenesis Burkholderia thailandensis
A172P random mutagenesis Burkholderia thailandensis
A172T random mutagenesis Burkholderia thailandensis
A172V random mutagenesis Burkholderia thailandensis
C69F random mutagenesis Burkholderia thailandensis
C69Y random mutagenesis Burkholderia thailandensis
D176G random mutagenesis Burkholderia thailandensis
D176N random mutagenesis Burkholderia thailandensis
D179N random mutagenesis Burkholderia thailandensis
E166D random mutagenesis Burkholderia thailandensis
E166G random mutagenesis Burkholderia thailandensis
E166K random mutagenesis Burkholderia thailandensis
E168del random mutagenesis Burkholderia thailandensis
L162F random mutagenesis Burkholderia thailandensis
L169P random mutagenesis Burkholderia thailandensis
L169Q random mutagenesis Burkholderia thailandensis
L169R random mutagenesis Burkholderia thailandensis
additional information large scale mutant screening, construction of four deletion mutations in a class A beta-lactamase PenA, each conferring an extended substrate spectrum. Single-amino-acid deletions T171del, I173del, and P174del and a two-amino-acid deletion, R165_T167delinsP, occurr in the omega loop, increasing the flexibility of the binding cavity. The four additional deletion mutations in PenA extend the substrate spectrum, in addition to the characterized E168del mutation. The mutations are potential future mutations conferring high-level ceftazidime resistance on isolates from clinical settings, compared with amino acid substitution mutations. MIC values of mutant enzymes for antibiotics ceftazidime, amoxicillin, amoxicillin-clavulanic acid, cefotaxime, ceftriaxone, cefepime, and meropenem, overview. None of the deletion mutations confer resistance to cefepime, a fourth-generation cephalosporin, or to meropenem, a carbapenem subgroup member, but the deletion mutations exhibit various profiles of resistance to expanded-spectrum cephalosporins Burkholderia thailandensis
N136D random mutagenesis Burkholderia thailandensis
N136K random mutagenesis Burkholderia thailandensis
N136T random mutagenesis Burkholderia thailandensis
N170H random mutagenesis Burkholderia thailandensis
N170K random mutagenesis Burkholderia thailandensis
N170Y random mutagenesis Burkholderia thailandensis
P174del random mutagenesis Burkholderia thailandensis
P174L random mutagenesis Burkholderia thailandensis
P174S random mutagenesis Burkholderia thailandensis
R163S random mutagenesis Burkholderia thailandensis
R164C random mutagenesis Burkholderia thailandensis
R164H random mutagenesis Burkholderia thailandensis
R164L random mutagenesis Burkholderia thailandensis
T171P random mutagenesis Burkholderia thailandensis

Organism

Organism UniProt Comment Textmining
Burkholderia thailandensis
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-
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Synonyms

Synonyms Comment Organism
class A beta-lactamase
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 Burkholderia thailandensis
PenA
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 Burkholderia thailandensis