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Literature summary for 3.5.2.6 extracted from
- Engineering allosteric regulation into the hinge region of a circularly permuted TEM-1 beta-lactamase (2010), Protein Eng. Des. Sel., 23, 699-709.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| M182T/T195S/A224V | mutant selected by a directed evolution strategy. It is allosterically downregulated by Zn2+, Ni2+ and Co2+ with binding affinities around 300 microM | Escherichia coli |
| R61H/E64H/43H | circularly permuted enzyme, created by rational design. Mutant shows little regulation upon metal ion binding except for a weak activation with Zn2+ | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
penicillin G | mutant R61H/E64H/43H, 25°C, pH not specified in the publication | Escherichia coli |  |
| 0.0568 | - |
penicillin G | mutant M182T/T195S/A224V, 25°C, pH not specified in the publication | Escherichia coli | |
| 0.136 | - |
penicillin G | wild-type, 25°C, pH not specified in the publication | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| penicillin G + H2O | - |
Escherichia coli | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TEM-1 | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.3 | - |
penicillin G | mutant R61H/E64H/43H, 25°C, pH not specified in the publication | Escherichia coli | |
| 24.8 | - |
penicillin G | mutant M182T/T195S/A224V, 25°C, pH not specified in the publication | Escherichia coli | |
| 129 | - |
penicillin G | wild-type, 25°C, pH not specified in the publication | Escherichia coli | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 210 | - |
penicillin G | mutant R61H/E64H/43H, 25°C, pH not specified in the publication | Escherichia coli | |
| 437 | - |
penicillin G | mutant M182T/T195S/A224V, 25°C, pH not specified in the publication | Escherichia coli | |
| 950 | - |
penicillin G | wild-type, 25°C, pH not specified in the publication | Escherichia coli | |
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