Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
M182T/T195S/A224V | mutant selected by a directed evolution strategy. It is allosterically downregulated by Zn2+, Ni2+ and Co2+ with binding affinities around 300 microM | Escherichia coli |
R61H/E64H/43H | circularly permuted enzyme, created by rational design. Mutant shows little regulation upon metal ion binding except for a weak activation with Zn2+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
penicillin G | mutant R61H/E64H/43H, 25°C, pH not specified in the publication | Escherichia coli | |
0.0568 | - |
penicillin G | mutant M182T/T195S/A224V, 25°C, pH not specified in the publication | Escherichia coli | |
0.136 | - |
penicillin G | wild-type, 25°C, pH not specified in the publication | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
penicillin G + H2O | - |
Escherichia coli | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TEM-1 | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.3 | - |
penicillin G | mutant R61H/E64H/43H, 25°C, pH not specified in the publication | Escherichia coli | |
24.8 | - |
penicillin G | mutant M182T/T195S/A224V, 25°C, pH not specified in the publication | Escherichia coli | |
129 | - |
penicillin G | wild-type, 25°C, pH not specified in the publication | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
210 | - |
penicillin G | mutant R61H/E64H/43H, 25°C, pH not specified in the publication | Escherichia coli | |
437 | - |
penicillin G | mutant M182T/T195S/A224V, 25°C, pH not specified in the publication | Escherichia coli | |
950 | - |
penicillin G | wild-type, 25°C, pH not specified in the publication | Escherichia coli |