Literature summary for 3.5.2.6 extracted from

Sekiguchi, J.; Morita, K.; Kitao, T.; Watanabe, N.; Okazaki, M.; Miyoshi-Akiyama, T.; Kanamori, M.; Kirikae, T.
KHM-1, a novel plasmid-mediated metallo-beta -lactamase from a Citrobacter freundii clinical isolate (2008), Antimicrob. Agents Chemother., 52, 4194-4197.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Citrobacter freundii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0044	-	cephaloridine	-	Citrobacter freundii
0.008	-	ceftazidime	-	Citrobacter freundii
0.012	-	meropenem	-	Citrobacter freundii
0.013	-	cefotaxime	-	Citrobacter freundii
0.071	-	moxalactam	-	Citrobacter freundii
0.081	-	cefoxitin	-	Citrobacter freundii
0.268	-	Imipenem	-	Citrobacter freundii
0.978	-	ampicillin	-	Citrobacter freundii
1.34	-	penicillin G	-	Citrobacter freundii

Organism

Organism	UniProt	Comment	Textmining
Citrobacter freundii	A8R403	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ampicillin + H2O	-	Citrobacter freundii	(2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?
cefotaxime + H2O	-	Citrobacter freundii	(2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
cefoxitin + H2O	-	Citrobacter freundii	(2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?
ceftazidime + H2O	-	Citrobacter freundii	(2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
cephaloridine + H2O	-	Citrobacter freundii	(2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate	-	?
imipenem + H2O	-	Citrobacter freundii	(5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
meropenem + H2O	-	Citrobacter freundii	(4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid	-	?
moxalactam + H2O	-	Citrobacter freundii	(2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid	-	?
penicillin G + H2O	-	Citrobacter freundii	(2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid	-	?

Synonyms

Synonyms	Comment	Organism
KHM-1	-	Citrobacter freundii
metallo-beta-lactamase	-	Citrobacter freundii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.4	-	meropenem	-	Citrobacter freundii
15	-	Imipenem	-	Citrobacter freundii
19	-	ampicillin	-	Citrobacter freundii
23	-	penicillin G	-	Citrobacter freundii
118	-	ceftazidime	-	Citrobacter freundii
686	-	cephaloridine	-	Citrobacter freundii
1178	-	cefoxitin	-	Citrobacter freundii
2181	-	cefotaxime	-	Citrobacter freundii
2794	-	moxalactam	-	Citrobacter freundii

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Release 2023.1 (January 2023)