Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Pseudomonas aeruginosa |
genes blaVIM1 and blaVIM-13, genetic structure and genotyping, VIM-13 is harbored in a class 1 integron, along with an A108T variant of the aminoglycoside-modifying enzyme encoding gene aacA4, which confers resistance to gentamicin and tobramycin | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-o-phenanthroline | - |
Pseudomonas aeruginosa | |
1,10-phenanthroline | - |
Pseudomonas aeruginosa | |
dipicolinic acid | - |
Pseudomonas aeruginosa | |
EDTA | EDTA inhibits the activity of VIM-13 approximately 25 times less than it inhibits the activity of VIM-1 | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
Imipenem | - |
Pseudomonas aeruginosa | |
0.004 | - |
Imipenem | VIM-1 | Pseudomonas aeruginosa | |
0.0155 | - |
meropenem | - |
Pseudomonas aeruginosa | |
0.0155 | - |
meropenem | VIM-13 | Pseudomonas aeruginosa | |
0.0185 | - |
Imipenem | - |
Pseudomonas aeruginosa | |
0.0185 | - |
Imipenem | VIM-13 | Pseudomonas aeruginosa | |
0.02 | - |
ampicillin | VIM-13 | Pseudomonas aeruginosa | |
0.056 | - |
cefuroxime | - |
Pseudomonas aeruginosa | |
0.056 | - |
cefuroxime | VIM-13 | Pseudomonas aeruginosa | |
0.072 | - |
meropenem | - |
Pseudomonas aeruginosa | |
0.072 | - |
meropenem | VIM-1 | Pseudomonas aeruginosa | |
0.076 | - |
cephalothin | - |
Pseudomonas aeruginosa | |
0.076 | - |
cephalothin | VIM-13 | Pseudomonas aeruginosa | |
0.135 | - |
cephalothin | - |
Pseudomonas aeruginosa | |
0.135 | - |
cephalothin | VIM-1 | Pseudomonas aeruginosa | |
0.148 | - |
cefuroxime | - |
Pseudomonas aeruginosa | |
0.148 | - |
cefuroxime | VIM-1 | Pseudomonas aeruginosa | |
0.197 | - |
ampicillin | - |
Pseudomonas aeruginosa | |
0.233 | - |
cefotaxime | - |
Pseudomonas aeruginosa | |
0.233 | - |
cefotaxime | VIM-13 | Pseudomonas aeruginosa | |
0.285 | - |
cefotaxime | - |
Pseudomonas aeruginosa | |
0.285 | - |
cefotaxime | VIM-1 | Pseudomonas aeruginosa | |
0.337 | - |
cefepime | - |
Pseudomonas aeruginosa | |
0.34 | - |
cefepime | VIM-1 | Pseudomonas aeruginosa | |
0.509 | - |
ceftazidime | - |
Pseudomonas aeruginosa | |
0.509 | - |
ceftazidime | VIM-13 | Pseudomonas aeruginosa | |
0.729 | - |
piperacillin | - |
Pseudomonas aeruginosa | |
0.729 | - |
piperacillin | VIM-13 | Pseudomonas aeruginosa | |
1.127 | - |
penicillin G | - |
Pseudomonas aeruginosa | |
1.127 | - |
penicillin G | VIM-13 | Pseudomonas aeruginosa | |
1.132 | - |
ceftazidime | - |
Pseudomonas aeruginosa | |
1.132 | - |
ceftazidime | VIM-1 | Pseudomonas aeruginosa | |
1.3 | - |
penicillin G | VIM-1 | Pseudomonas aeruginosa | |
1.303 | - |
penicillin G | - |
Pseudomonas aeruginosa | |
1.377 | - |
piperacillin | - |
Pseudomonas aeruginosa | |
1.38 | - |
piperacillin | VIM-1 | Pseudomonas aeruginosa | |
1.737 | - |
ampicillin | - |
Pseudomonas aeruginosa | |
1.74 | - |
ampicillin | VIM-1 | Pseudomonas aeruginosa | |
1.87 | - |
cefepime | - |
Pseudomonas aeruginosa | |
1.87 | - |
cefepime | VIM-13 | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | - |
Pseudomonas aeruginosa | |
Zn2+ | zinc-containing metalloenzyme | Pseudomonas aeruginosa | |
Zn2+ | VIM metallo-beta-lactamases, VIM-1 and VIM-13 inactivation by Zn2+ removal | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
SDS-PAGE | Pseudomonas aeruginosa |
28220 | - |
SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas aeruginosa | screening for beta-lactamase genes in clincal isolates using Etest MBL screening test, The blaVIM-13-producing isolate shows resistance to all beta-lactams, except aztreonam, gentamicin, tobramycin, and ciprofloxacin, VIM-13 confers slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime than VIM-1, MIC values, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
clinical human isolates from Majorca, Spain, e.g. strain PA-SL2, and strain PAO1, genes blaVIM-1 and blaVIM-13 | - |
Pseudomonas aeruginosa | Q2HY42 | SL2 | - |
Pseudomonas aeruginosa | Q9XAY4 | SL2 | - |
Pseudomonas aeruginosa SL2 | Q2HY42 | SL2 | - |
Pseudomonas aeruginosa SL2 | Q9XAY4 | SL2 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Pseudomonas aeruginosa SL2 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefepime + H2O | - |
Pseudomonas aeruginosa | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefepime + H2O | - |
Pseudomonas aeruginosa SL2 | (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefotaxime + H2O | - |
Pseudomonas aeruginosa | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefotaxime + H2O | - |
Pseudomonas aeruginosa SL2 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | - |
Pseudomonas aeruginosa | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cefuroxime + H2O | - |
Pseudomonas aeruginosa | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefuroxime + H2O | - |
Pseudomonas aeruginosa SL2 | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalothin + H2O | - |
Pseudomonas aeruginosa | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalothin + H2O | - |
Pseudomonas aeruginosa SL2 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
imipenem + H2O | - |
Pseudomonas aeruginosa | (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
meropenem + H2O | - |
Pseudomonas aeruginosa | (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid | - |
? | |
additional information | screening for beta-lactamase genes in clincal isolates using Etest MBL screening test, The blaVIM-13-producing isolate shows resistance to all beta-lactams, except aztreonam, gentamicin, tobramycin, and ciprofloxacin, VIM-13 confers slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime than VIM-1, MIC values, overview | Pseudomonas aeruginosa | ? | - |
? | |
penicillin G + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Pseudomonas aeruginosa | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 26000, SDS-PAGE | Pseudomonas aeruginosa |
? | x * 28220, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
carbapenemase | - |
Pseudomonas aeruginosa |
class B carbapenemase | - |
Pseudomonas aeruginosa |
MBL | - |
Pseudomonas aeruginosa |
metallo-beta-lactamase | - |
Pseudomonas aeruginosa |
VIM-1 | - |
Pseudomonas aeruginosa |
VIM-13 | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
Imipenem | - |
Pseudomonas aeruginosa | |
3.4 | - |
Imipenem | VIM-1 | Pseudomonas aeruginosa | |
9 | - |
meropenem | - |
Pseudomonas aeruginosa | |
9 | - |
meropenem | VIM-13 | Pseudomonas aeruginosa | |
10 | - |
ceftazidime | - |
Pseudomonas aeruginosa | |
10 | - |
ceftazidime | VIM-13 | Pseudomonas aeruginosa | |
20 | - |
meropenem | - |
Pseudomonas aeruginosa | |
20 | - |
meropenem | VIM-1 | Pseudomonas aeruginosa | |
42 | - |
ceftazidime | - |
Pseudomonas aeruginosa | |
42 | - |
ceftazidime | VIM-1 | Pseudomonas aeruginosa | |
54 | - |
Imipenem | - |
Pseudomonas aeruginosa | |
54 | - |
Imipenem | VIM-13 | Pseudomonas aeruginosa | |
61 | - |
cefepime | - |
Pseudomonas aeruginosa | |
61 | - |
cefepime | VIM-13 | Pseudomonas aeruginosa | |
67 | - |
ampicillin | - |
Pseudomonas aeruginosa | |
67 | - |
ampicillin | VIM-13 | Pseudomonas aeruginosa | |
119 | - |
cefepime | - |
Pseudomonas aeruginosa | |
119 | - |
cefepime | VIM-1 | Pseudomonas aeruginosa | |
204 | - |
cefotaxime | - |
Pseudomonas aeruginosa | |
204 | - |
cefotaxime | VIM-1 | Pseudomonas aeruginosa | |
239 | - |
cefuroxime | - |
Pseudomonas aeruginosa | |
239 | - |
cefuroxime | VIM-1 | Pseudomonas aeruginosa | |
283 | - |
cefuroxime | - |
Pseudomonas aeruginosa | |
283 | - |
cefuroxime | VIM-13 | Pseudomonas aeruginosa | |
307 | - |
ampicillin | - |
Pseudomonas aeruginosa | |
307 | - |
ampicillin | VIM-1 | Pseudomonas aeruginosa | |
362 | - |
piperacillin | - |
Pseudomonas aeruginosa | |
362 | - |
piperacillin | VIM-13 | Pseudomonas aeruginosa | |
374 | - |
piperacillin | - |
Pseudomonas aeruginosa | |
374 | - |
piperacillin | VIM-1 | Pseudomonas aeruginosa | |
418 | - |
penicillin G | - |
Pseudomonas aeruginosa | |
418 | - |
penicillin G | VIM-1 | Pseudomonas aeruginosa | |
449 | - |
cephalothin | - |
Pseudomonas aeruginosa | |
449 | - |
cephalothin | VIM-1 | Pseudomonas aeruginosa | |
612 | - |
cefotaxime | - |
Pseudomonas aeruginosa | |
612 | - |
cefotaxime | VIM-13 | Pseudomonas aeruginosa | |
656 | - |
cephalothin | - |
Pseudomonas aeruginosa | |
656 | - |
cephalothin | VIM-13 | Pseudomonas aeruginosa | |
757 | - |
penicillin G | - |
Pseudomonas aeruginosa | |
757 | - |
penicillin G | VIM-13 | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric focusing | - |
5.1 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0093 | - |
VIM-1 | Pseudomonas aeruginosa | EDTA | |
0.0093 | - |
imipenem as substrate | Pseudomonas aeruginosa | EDTA | |
0.0416 | - |
VIM-1 | Pseudomonas aeruginosa | dipicolinic acid | |
0.0416 | - |
imipenem as substrate | Pseudomonas aeruginosa | dipicolinic acid | |
0.048 | - |
VIM-13 | Pseudomonas aeruginosa | dipicolinic acid | |
0.048 | - |
imipenem as substrate | Pseudomonas aeruginosa | dipicolinic acid | |
0.0693 | - |
VIM-13 | Pseudomonas aeruginosa | 1,10-o-phenanthroline | |
0.073 | - |
VIM-1 | Pseudomonas aeruginosa | 1,10-o-phenanthroline | |
0.073 | - |
imipenem as substrate | Pseudomonas aeruginosa | 1,10-phenanthroline | |
0.253 | - |
VIM-13 | Pseudomonas aeruginosa | EDTA | |
0.693 | - |
imipenem as substrate | Pseudomonas aeruginosa | 1,10-phenanthroline | |
2.526 | - |
imipenem as substrate | Pseudomonas aeruginosa | EDTA |