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Literature summary for 3.5.2.6 extracted from

  • Juan, C.; Beceiro, A.; Gutiérrez, O.; Albertí, S.; Garau, M.; Pérez, J.L.; Bou, G.; Oliver, A.
    Characterization of the new metallo-beta-lactamase VIM-13 and its integron-borne gene from a Pseudomonas aeruginosa clinical isolate in Spain (2008), Antimicrob. Agents Chemother., 52, 3589-3596.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Pseudomonas aeruginosa
genes blaVIM1 and blaVIM-13, genetic structure and genotyping, VIM-13 is harbored in a class 1 integron, along with an A108T variant of the aminoglycoside-modifying enzyme encoding gene aacA4, which confers resistance to gentamicin and tobramycin Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
1,10-o-phenanthroline
-
Pseudomonas aeruginosa
1,10-phenanthroline
-
Pseudomonas aeruginosa
dipicolinic acid
-
Pseudomonas aeruginosa
EDTA EDTA inhibits the activity of VIM-13 approximately 25 times less than it inhibits the activity of VIM-1 Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
Imipenem
-
Pseudomonas aeruginosa
0.004
-
Imipenem VIM-1 Pseudomonas aeruginosa
0.0155
-
meropenem
-
Pseudomonas aeruginosa
0.0155
-
meropenem VIM-13 Pseudomonas aeruginosa
0.0185
-
Imipenem
-
Pseudomonas aeruginosa
0.0185
-
Imipenem VIM-13 Pseudomonas aeruginosa
0.02
-
ampicillin VIM-13 Pseudomonas aeruginosa
0.056
-
cefuroxime
-
Pseudomonas aeruginosa
0.056
-
cefuroxime VIM-13 Pseudomonas aeruginosa
0.072
-
meropenem
-
Pseudomonas aeruginosa
0.072
-
meropenem VIM-1 Pseudomonas aeruginosa
0.076
-
cephalothin
-
Pseudomonas aeruginosa
0.076
-
cephalothin VIM-13 Pseudomonas aeruginosa
0.135
-
cephalothin
-
Pseudomonas aeruginosa
0.135
-
cephalothin VIM-1 Pseudomonas aeruginosa
0.148
-
cefuroxime
-
Pseudomonas aeruginosa
0.148
-
cefuroxime VIM-1 Pseudomonas aeruginosa
0.197
-
ampicillin
-
Pseudomonas aeruginosa
0.233
-
cefotaxime
-
Pseudomonas aeruginosa
0.233
-
cefotaxime VIM-13 Pseudomonas aeruginosa
0.285
-
cefotaxime
-
Pseudomonas aeruginosa
0.285
-
cefotaxime VIM-1 Pseudomonas aeruginosa
0.337
-
cefepime
-
Pseudomonas aeruginosa
0.34
-
cefepime VIM-1 Pseudomonas aeruginosa
0.509
-
ceftazidime
-
Pseudomonas aeruginosa
0.509
-
ceftazidime VIM-13 Pseudomonas aeruginosa
0.729
-
piperacillin
-
Pseudomonas aeruginosa
0.729
-
piperacillin VIM-13 Pseudomonas aeruginosa
1.127
-
penicillin G
-
Pseudomonas aeruginosa
1.127
-
penicillin G VIM-13 Pseudomonas aeruginosa
1.132
-
ceftazidime
-
Pseudomonas aeruginosa
1.132
-
ceftazidime VIM-1 Pseudomonas aeruginosa
1.3
-
penicillin G VIM-1 Pseudomonas aeruginosa
1.303
-
penicillin G
-
Pseudomonas aeruginosa
1.377
-
piperacillin
-
Pseudomonas aeruginosa
1.38
-
piperacillin VIM-1 Pseudomonas aeruginosa
1.737
-
ampicillin
-
Pseudomonas aeruginosa
1.74
-
ampicillin VIM-1 Pseudomonas aeruginosa
1.87
-
cefepime
-
Pseudomonas aeruginosa
1.87
-
cefepime VIM-13 Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Pseudomonas aeruginosa
Zn2+ zinc-containing metalloenzyme Pseudomonas aeruginosa
Zn2+ VIM metallo-beta-lactamases, VIM-1 and VIM-13 inactivation by Zn2+ removal Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
SDS-PAGE Pseudomonas aeruginosa
28220
-
SDS-PAGE Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa screening for beta-lactamase genes in clincal isolates using Etest MBL screening test, The blaVIM-13-producing isolate shows resistance to all beta-lactams, except aztreonam, gentamicin, tobramycin, and ciprofloxacin, VIM-13 confers slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime than VIM-1, MIC values, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
clinical human isolates from Majorca, Spain, e.g. strain PA-SL2, and strain PAO1, genes blaVIM-1 and blaVIM-13
-
Pseudomonas aeruginosa Q2HY42 SL2
-
Pseudomonas aeruginosa Q9XAY4 SL2
-
Pseudomonas aeruginosa SL2 Q2HY42 SL2
-
Pseudomonas aeruginosa SL2 Q9XAY4 SL2
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
Pseudomonas aeruginosa (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
ampicillin + H2O
-
Pseudomonas aeruginosa SL2 (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cefepime + H2O
-
Pseudomonas aeruginosa (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefepime + H2O
-
Pseudomonas aeruginosa SL2 (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefotaxime + H2O
-
Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefotaxime + H2O
-
Pseudomonas aeruginosa SL2 (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
ceftazidime + H2O
-
Pseudomonas aeruginosa (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cefuroxime + H2O
-
Pseudomonas aeruginosa (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cefuroxime + H2O
-
Pseudomonas aeruginosa SL2 (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cephalothin + H2O
-
Pseudomonas aeruginosa (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cephalothin + H2O
-
Pseudomonas aeruginosa SL2 (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
imipenem + H2O
-
Pseudomonas aeruginosa (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
meropenem + H2O
-
Pseudomonas aeruginosa (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
?
additional information screening for beta-lactamase genes in clincal isolates using Etest MBL screening test, The blaVIM-13-producing isolate shows resistance to all beta-lactams, except aztreonam, gentamicin, tobramycin, and ciprofloxacin, VIM-13 confers slightly higher levels of resistance to piperacillin and lower levels of resistance to ceftazidime and cefepime than VIM-1, MIC values, overview Pseudomonas aeruginosa ?
-
?
penicillin G + H2O
-
Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
piperacillin + H2O
-
Pseudomonas aeruginosa (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Pseudomonas aeruginosa
? x * 28220, SDS-PAGE Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
carbapenemase
-
Pseudomonas aeruginosa
class B carbapenemase
-
Pseudomonas aeruginosa
MBL
-
Pseudomonas aeruginosa
metallo-beta-lactamase
-
Pseudomonas aeruginosa
VIM-1
-
Pseudomonas aeruginosa
VIM-13
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
Imipenem
-
Pseudomonas aeruginosa
3.4
-
Imipenem VIM-1 Pseudomonas aeruginosa
9
-
meropenem
-
Pseudomonas aeruginosa
9
-
meropenem VIM-13 Pseudomonas aeruginosa
10
-
ceftazidime
-
Pseudomonas aeruginosa
10
-
ceftazidime VIM-13 Pseudomonas aeruginosa
20
-
meropenem
-
Pseudomonas aeruginosa
20
-
meropenem VIM-1 Pseudomonas aeruginosa
42
-
ceftazidime
-
Pseudomonas aeruginosa
42
-
ceftazidime VIM-1 Pseudomonas aeruginosa
54
-
Imipenem
-
Pseudomonas aeruginosa
54
-
Imipenem VIM-13 Pseudomonas aeruginosa
61
-
cefepime
-
Pseudomonas aeruginosa
61
-
cefepime VIM-13 Pseudomonas aeruginosa
67
-
ampicillin
-
Pseudomonas aeruginosa
67
-
ampicillin VIM-13 Pseudomonas aeruginosa
119
-
cefepime
-
Pseudomonas aeruginosa
119
-
cefepime VIM-1 Pseudomonas aeruginosa
204
-
cefotaxime
-
Pseudomonas aeruginosa
204
-
cefotaxime VIM-1 Pseudomonas aeruginosa
239
-
cefuroxime
-
Pseudomonas aeruginosa
239
-
cefuroxime VIM-1 Pseudomonas aeruginosa
283
-
cefuroxime
-
Pseudomonas aeruginosa
283
-
cefuroxime VIM-13 Pseudomonas aeruginosa
307
-
ampicillin
-
Pseudomonas aeruginosa
307
-
ampicillin VIM-1 Pseudomonas aeruginosa
362
-
piperacillin
-
Pseudomonas aeruginosa
362
-
piperacillin VIM-13 Pseudomonas aeruginosa
374
-
piperacillin
-
Pseudomonas aeruginosa
374
-
piperacillin VIM-1 Pseudomonas aeruginosa
418
-
penicillin G
-
Pseudomonas aeruginosa
418
-
penicillin G VIM-1 Pseudomonas aeruginosa
449
-
cephalothin
-
Pseudomonas aeruginosa
449
-
cephalothin VIM-1 Pseudomonas aeruginosa
612
-
cefotaxime
-
Pseudomonas aeruginosa
612
-
cefotaxime VIM-13 Pseudomonas aeruginosa
656
-
cephalothin
-
Pseudomonas aeruginosa
656
-
cephalothin VIM-13 Pseudomonas aeruginosa
757
-
penicillin G
-
Pseudomonas aeruginosa
757
-
penicillin G VIM-13 Pseudomonas aeruginosa

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas aeruginosa isoelectric focusing
-
5.1

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0093
-
VIM-1 Pseudomonas aeruginosa EDTA
0.0093
-
imipenem as substrate Pseudomonas aeruginosa EDTA
0.0416
-
VIM-1 Pseudomonas aeruginosa dipicolinic acid
0.0416
-
imipenem as substrate Pseudomonas aeruginosa dipicolinic acid
0.048
-
VIM-13 Pseudomonas aeruginosa dipicolinic acid
0.048
-
imipenem as substrate Pseudomonas aeruginosa dipicolinic acid
0.0693
-
VIM-13 Pseudomonas aeruginosa 1,10-o-phenanthroline
0.073
-
VIM-1 Pseudomonas aeruginosa 1,10-o-phenanthroline
0.073
-
imipenem as substrate Pseudomonas aeruginosa 1,10-phenanthroline
0.253
-
VIM-13 Pseudomonas aeruginosa EDTA
0.693
-
imipenem as substrate Pseudomonas aeruginosa 1,10-phenanthroline
2.526
-
imipenem as substrate Pseudomonas aeruginosa EDTA