Literature summary for 3.5.2.6 extracted from

Nauton, L.; Kahn, R.; Garau, G.; Hernandez, J.F.; Dideberg, O.
Structural insights into the design of inhibitors for the L1 metallo-beta-lactamase from Stenotrophomonas maltophilia (2008), J. Mol. Biol., 375, 257-269.

Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain BL21 Star (DE3)	Stenotrophomonas maltophilia

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapour diffusion method with 100 mM HEPES (pH 7.75), 1.8 M (NH4)2SO4, 1.5% (v/v) PEG400	Stenotrophomonas maltophilia

Inhibitors

Inhibitors	Comment	Organism	Structure
1-(2S)-3-mercapto-2-methyl-propionyl-D-proline	competitive inhibitor	Stenotrophomonas maltophilia
2,5-diphenyl-2H-pyrazol-3,4-dicarboxylic acid	-	Stenotrophomonas maltophilia
2,5-diphenyl-3,4-furan dicarboxylic acid	-	Stenotrophomonas maltophilia
2-(((3-mercapto-5-(2-methylphenyl)-4H-1,2,4-triazol-4-yl)imino)methyl)benzoic acid	most effective inhibitor	Stenotrophomonas maltophilia
N-[(benzyloxy) carbonyl]-L-cysteinyl glycine	-	Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	dependent	Stenotrophomonas maltophilia

Organism

Organism	UniProt	Comment	Textmining
Stenotrophomonas maltophilia	P52700	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q Sepharose column chromatography, SP-Sepharose column chromatography, and gel filtration	Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
nitrocefin + H2O	-	Stenotrophomonas maltophilia	(2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid	-	?

Subunits

Subunits	Comment	Organism
tetramer	x-ray crystallography	Stenotrophomonas maltophilia

Synonyms

Synonyms	Comment	Organism
L1 metallo-beta-lactamase	-	Stenotrophomonas maltophilia

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Release 2023.1 (January 2023)