Protein Variants | Comment | Organism |
---|---|---|
D170N | increase in Ki-value for clavulanic acid, little difference in the hydrolytic profile of beta-lactams compared to wild-type,no ceftazidime hydrolysis | Burkholderia pseudomallei |
K232N | increase in Ki-value for clavulanic acid, little difference in the hydrolytic profile of beta-lactams compared to wild-type | Burkholderia pseudomallei |
S104P | mutant enzyme shows no beta-lactam hydrolytic activity | Burkholderia pseudomallei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
clavulanic acid | competitive | Burkholderia pseudomallei | |
additional information | no inhibition of wild-type enzyme and mutant enzymes D170N and K232N by NaCl up to 1 M | Burkholderia pseudomallei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00948 | - |
piperacillin | mutant enzyme K232N | Burkholderia pseudomallei | |
0.0132 | - |
nitrocefin | mutant enzyme K232N | Burkholderia pseudomallei | |
0.0213 | - |
piperacillin | wild-type enzyme | Burkholderia pseudomallei | |
0.0221 | - |
piperacillin | mutant enzyme D170N | Burkholderia pseudomallei | |
0.0287 | - |
Cloxacillin | wild-type enzyme | Burkholderia pseudomallei | |
0.0295 | - |
penicillin G | wild-type enzyme | Burkholderia pseudomallei | |
0.0372 | - |
Oxacillin | mutant enzyme D170N | Burkholderia pseudomallei | |
0.0389 | - |
ampicillin | mutant enzyme D170N | Burkholderia pseudomallei | |
0.0407 | - |
Oxacillin | wild-type enzyme | Burkholderia pseudomallei | |
0.0466 | - |
penicillin G | mutant enzyme K232N | Burkholderia pseudomallei | |
0.0504 | - |
nitrocefin | mutant enzyme D170N | Burkholderia pseudomallei | |
0.0554 | - |
cephalothin | mutant enzyme K232N | Burkholderia pseudomallei | |
0.0563 | - |
penicillin G | mutant enzyme D170N | Burkholderia pseudomallei | |
0.0719 | - |
ampicillin | wild-type enzyme | Burkholderia pseudomallei | |
0.0979 | - |
cephaloridine | wild-type enzyme | Burkholderia pseudomallei | |
0.1 | - |
cephalothin | mutant enzyme D170N | Burkholderia pseudomallei | |
0.157 | - |
ampicillin | mutant enzyme K232N | Burkholderia pseudomallei | |
0.163 | - |
cephalothin | wild-type enzyme | Burkholderia pseudomallei | |
0.166 | - |
Cloxacillin | mutant enzyme D170N | Burkholderia pseudomallei | |
0.179 | - |
nitrocefin | wild-type enzyme | Burkholderia pseudomallei | |
0.18 | - |
Cloxacillin | mutant enzyme K232N | Burkholderia pseudomallei | |
0.194 | - |
Oxacillin | mutant enzyme K232N | Burkholderia pseudomallei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia pseudomallei | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Burkholderia pseudomallei | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | - |
Burkholderia pseudomallei | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
Burkholderia pseudomallei | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cloxacillin + H2O | - |
Burkholderia pseudomallei | (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Burkholderia pseudomallei | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
oxacillin + H2O | - |
Burkholderia pseudomallei | (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
penicillin G + H2O | - |
Burkholderia pseudomallei | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
piperacillin + H2O | - |
Burkholderia pseudomallei | (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OXA-57 | - |
Burkholderia pseudomallei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.28 | - |
cephalothin | wild-type enzyme | Burkholderia pseudomallei | |
0.4 | - |
penicillin G | wild-type enzyme | Burkholderia pseudomallei | |
0.51 | - |
Cloxacillin | wild-type enzyme | Burkholderia pseudomallei | |
0.54 | - |
ampicillin | wild-type enzyme | Burkholderia pseudomallei | |
0.61 | - |
penicillin G | mutant enzyme D170N | Burkholderia pseudomallei | |
0.65 | - |
cephalothin | mutant enzyme K232N | Burkholderia pseudomallei | |
0.75 | - |
penicillin G | mutant enzyme K232N | Burkholderia pseudomallei | |
0.86 | - |
cephalothin | mutant enzyme D170N | Burkholderia pseudomallei | |
1.04 | - |
Cloxacillin | mutant enzyme K232N | Burkholderia pseudomallei | |
1.12 | - |
Cloxacillin | mutant enzyme D170N | Burkholderia pseudomallei | |
1.23 | - |
Oxacillin | wild-type enzyme | Burkholderia pseudomallei | |
1.42 | - |
ampicillin | mutant enzyme K232N | Burkholderia pseudomallei | |
2.11 | - |
piperacillin | wild-type enzyme | Burkholderia pseudomallei | |
2.12 | - |
ampicillin | mutant enzyme D170N | Burkholderia pseudomallei | |
2.37 | - |
Oxacillin | mutant enzyme D170N | Burkholderia pseudomallei | |
3.07 | - |
Oxacillin | mutant enzyme K232N | Burkholderia pseudomallei | |
4.31 | - |
piperacillin | mutant enzyme K232N | Burkholderia pseudomallei | |
5.7 | - |
piperacillin | mutant enzyme D170N | Burkholderia pseudomallei | |
13 | - |
cephaloridine | wild-type enzyme | Burkholderia pseudomallei | |
29.7 | - |
nitrocefin | mutant enzyme K232N | Burkholderia pseudomallei | |
63 | - |
nitrocefin | wild-type enzyme | Burkholderia pseudomallei | |
79.5 | - |
nitrocefin | mutant enzyme D170N | Burkholderia pseudomallei |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
3.4 | - |
clavulanic acid | wild-type enzyme | Burkholderia pseudomallei | |
3.9 | - |
clavulanic acid | mutant enzyme K232N | Burkholderia pseudomallei | |
4.6 | - |
clavulanic acid | mutant enzyme D170N | Burkholderia pseudomallei |