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Literature summary for 3.5.2.6 extracted from

  • Keith, K.E.; Oyston, P.C.; Crossett, B.; Fairweather, N.F.; Titball, R.W.; Walsh, T.R.; Brown, K.A.
    Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei (2005), Antimicrob. Agents Chemother., 49, 1639-1641.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
D170N increase in Ki-value for clavulanic acid, little difference in the hydrolytic profile of beta-lactams compared to wild-type,no ceftazidime hydrolysis Burkholderia pseudomallei
K232N increase in Ki-value for clavulanic acid, little difference in the hydrolytic profile of beta-lactams compared to wild-type Burkholderia pseudomallei
S104P mutant enzyme shows no beta-lactam hydrolytic activity Burkholderia pseudomallei

Inhibitors

Inhibitors Comment Organism Structure
clavulanic acid competitive Burkholderia pseudomallei
additional information no inhibition of wild-type enzyme and mutant enzymes D170N and K232N by NaCl up to 1 M Burkholderia pseudomallei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00948
-
piperacillin mutant enzyme K232N Burkholderia pseudomallei
0.0132
-
nitrocefin mutant enzyme K232N Burkholderia pseudomallei
0.0213
-
piperacillin wild-type enzyme Burkholderia pseudomallei
0.0221
-
piperacillin mutant enzyme D170N Burkholderia pseudomallei
0.0287
-
Cloxacillin wild-type enzyme Burkholderia pseudomallei
0.0295
-
penicillin G wild-type enzyme Burkholderia pseudomallei
0.0372
-
Oxacillin mutant enzyme D170N Burkholderia pseudomallei
0.0389
-
ampicillin mutant enzyme D170N Burkholderia pseudomallei
0.0407
-
Oxacillin wild-type enzyme Burkholderia pseudomallei
0.0466
-
penicillin G mutant enzyme K232N Burkholderia pseudomallei
0.0504
-
nitrocefin mutant enzyme D170N Burkholderia pseudomallei
0.0554
-
cephalothin mutant enzyme K232N Burkholderia pseudomallei
0.0563
-
penicillin G mutant enzyme D170N Burkholderia pseudomallei
0.0719
-
ampicillin wild-type enzyme Burkholderia pseudomallei
0.0979
-
cephaloridine wild-type enzyme Burkholderia pseudomallei
0.1
-
cephalothin mutant enzyme D170N Burkholderia pseudomallei
0.157
-
ampicillin mutant enzyme K232N Burkholderia pseudomallei
0.163
-
cephalothin wild-type enzyme Burkholderia pseudomallei
0.166
-
Cloxacillin mutant enzyme D170N Burkholderia pseudomallei
0.179
-
nitrocefin wild-type enzyme Burkholderia pseudomallei
0.18
-
Cloxacillin mutant enzyme K232N Burkholderia pseudomallei
0.194
-
Oxacillin mutant enzyme K232N Burkholderia pseudomallei

Organism

Organism UniProt Comment Textmining
Burkholderia pseudomallei
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
Burkholderia pseudomallei (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
cephaloridine + H2O
-
Burkholderia pseudomallei (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
?
cephalothin + H2O
-
Burkholderia pseudomallei (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
cloxacillin + H2O
-
Burkholderia pseudomallei (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
nitrocefin + H2O
-
Burkholderia pseudomallei (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
oxacillin + H2O
-
Burkholderia pseudomallei (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
penicillin G + H2O
-
Burkholderia pseudomallei (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
piperacillin + H2O
-
Burkholderia pseudomallei (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Synonyms

Synonyms Comment Organism
OXA-57
-
Burkholderia pseudomallei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.28
-
cephalothin wild-type enzyme Burkholderia pseudomallei
0.4
-
penicillin G wild-type enzyme Burkholderia pseudomallei
0.51
-
Cloxacillin wild-type enzyme Burkholderia pseudomallei
0.54
-
ampicillin wild-type enzyme Burkholderia pseudomallei
0.61
-
penicillin G mutant enzyme D170N Burkholderia pseudomallei
0.65
-
cephalothin mutant enzyme K232N Burkholderia pseudomallei
0.75
-
penicillin G mutant enzyme K232N Burkholderia pseudomallei
0.86
-
cephalothin mutant enzyme D170N Burkholderia pseudomallei
1.04
-
Cloxacillin mutant enzyme K232N Burkholderia pseudomallei
1.12
-
Cloxacillin mutant enzyme D170N Burkholderia pseudomallei
1.23
-
Oxacillin wild-type enzyme Burkholderia pseudomallei
1.42
-
ampicillin mutant enzyme K232N Burkholderia pseudomallei
2.11
-
piperacillin wild-type enzyme Burkholderia pseudomallei
2.12
-
ampicillin mutant enzyme D170N Burkholderia pseudomallei
2.37
-
Oxacillin mutant enzyme D170N Burkholderia pseudomallei
3.07
-
Oxacillin mutant enzyme K232N Burkholderia pseudomallei
4.31
-
piperacillin mutant enzyme K232N Burkholderia pseudomallei
5.7
-
piperacillin mutant enzyme D170N Burkholderia pseudomallei
13
-
cephaloridine wild-type enzyme Burkholderia pseudomallei
29.7
-
nitrocefin mutant enzyme K232N Burkholderia pseudomallei
63
-
nitrocefin wild-type enzyme Burkholderia pseudomallei
79.5
-
nitrocefin mutant enzyme D170N Burkholderia pseudomallei

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4
-
clavulanic acid wild-type enzyme Burkholderia pseudomallei
3.9
-
clavulanic acid mutant enzyme K232N Burkholderia pseudomallei
4.6
-
clavulanic acid mutant enzyme D170N Burkholderia pseudomallei