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Literature summary for 3.5.2.6 extracted from

  • Docquier, J.D.; Lopizzo, T.; Liberatori, S.; Prenna, M.; Thaller, M.C.; Frere, J.M.; Rossolini, G.M.
    Biochemical characterization of the THIN-B metallo-beta-lactamase of Janthinobacterium lividum (2004), Antimicrob. Agents Chemother., 48, 4778-4783.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Janthinobacterium lividum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
 Janthinobacterium lividum
dipicolinic acid
-
 Janthinobacterium lividum
EDTA
-
 Janthinobacterium lividum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 cefotaxime 30°C, pH 7.5 Janthinobacterium lividum
0.04
-
 meropenem 30°C, pH 7.5 Janthinobacterium lividum
0.05
-
 cefuroxime 30°C, pH 7.5 Janthinobacterium lividum
0.08
-
 Imipenem 30°C, pH 7.5 Janthinobacterium lividum
0.14
-
 ceftazidime 30°C, pH 7.5 Janthinobacterium lividum
0.5
-
 piperacillin 30°C, pH 7.5 Janthinobacterium lividum
1.3
-
 ampicillin 30°C, pH 7.5 Janthinobacterium lividum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30915
-
 x * 30915, MALDI-TOF mass spectrometry Janthinobacterium lividum

Organism

Organism UniProt Comment Textmining
Janthinobacterium lividum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Janthinobacterium lividum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.5
-
-
 Janthinobacterium lividum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Janthinobacterium lividum (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Janthinobacterium lividum (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefotaxime + H2O
-
 Janthinobacterium lividum (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
ceftazidime + H2O
-
 Janthinobacterium lividum (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefuroxime + H2O
-
 Janthinobacterium lividum (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
imipenem + H2O
-
 Janthinobacterium lividum (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
meropenem + H2O
-
 Janthinobacterium lividum (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
piperacillin + H2O
-
 Janthinobacterium lividum (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 30915, MALDI-TOF mass spectrometry Janthinobacterium lividum

Synonyms

Synonyms Comment Organism
THIN-B metallo-beta-lactamase
-
 Janthinobacterium lividum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
20
-
 ceftazidime 30°C, pH 7.5 Janthinobacterium lividum
80
-
 cefotaxime 30°C, pH 7.5 Janthinobacterium lividum
100
-
 piperacillin 30°C, pH 7.5 Janthinobacterium lividum
120
-
 Imipenem 30°C, pH 7.5 Janthinobacterium lividum
140
-
 cefuroxime 30°C, pH 7.5 Janthinobacterium lividum
200
-
 meropenem 30°C, pH 7.5 Janthinobacterium lividum
480
-
 ampicillin 30°C, pH 7.5 Janthinobacterium lividum