Cloned (Comment) | Organism |
---|---|
Escherichia coli strain HB101 is used as the host for the recombinant plasmids | Providencia stuartii |
Protein Variants | Comment | Organism |
---|---|---|
K104E | thermal stability is increased, mutant enzyme loses activity against oxyimino-cephgalosporins, aztreonam, and ampicillin | Providencia stuartii |
P51L | thermal stability is greatly decreased, mutant enzyme loses activity against cefotaxime and exhibits a reduced catalytic efficiency with penicillins and aztreonam, no inhibition by beta-odopenicillanate | Providencia stuartii |
S164E | thermal stability is increased, mutant enzyme loses activity against ceftazidime | Providencia stuartii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-iodopenicillanate | - |
Providencia stuartii | |
tazobactam | - |
Providencia stuartii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
penicillin G | mutant enzyme K104E | Providencia stuartii | |
0.0042 | - |
ampicillin | mutant enzyme K104E | Providencia stuartii | |
0.009 | - |
penicillin G | wild-type enzyme | Providencia stuartii | |
0.009 | - |
ampicillin | wild-type enzyme | Providencia stuartii | |
0.015 | - |
penicillin G | mutant enzyme S164R | Providencia stuartii | |
0.018 | - |
cephaloridine | mutant enzyme P51L | Providencia stuartii | |
0.02 | - |
nitrocefin | wild-type enzyme | Providencia stuartii | |
0.028 | - |
cephaloridine | wild-type enzyme | Providencia stuartii | |
0.031 | - |
(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | wild-type enzyme | Providencia stuartii | |
0.041 | - |
nitrocefin | mutant enzyme K104E | Providencia stuartii | |
0.055 | - |
aztreonam | wild-type enzyme | Providencia stuartii | |
0.059 | - |
ceftazidime | wild-type enzyme | Providencia stuartii | |
0.065 | - |
nitrocefin | mutant enzyme S164R | Providencia stuartii | |
0.07 | - |
cephaloridine | mutant enzyme K104E | Providencia stuartii | |
0.08 | - |
ceftazidime | mutant enzyme P51L | Providencia stuartii | |
0.156 | - |
cephaloridine | mutant enzyme S164R | Providencia stuartii | |
0.18 | - |
nitrocefin | mutant enzyme P51L | Providencia stuartii | |
0.21 | - |
(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | mutant enzyme S164R | Providencia stuartii | |
0.335 | - |
ampicillin | mutant enzyme S164R | Providencia stuartii | |
0.45 | - |
ampicillin | mutant enzyme P51L | Providencia stuartii | |
0.575 | - |
penicillin G | mutant enzyme P51L | Providencia stuartii | |
0.66 | - |
aztreonam | mutant enzyme P51L | Providencia stuartii | |
1.66 | - |
aztreonam | mutant enzyme S164R | Providencia stuartii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia stuartii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Providencia stuartii | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
aztreonam + H2O | - |
Providencia stuartii | [(1S,2S)-1-[[(2Z)-2-(2-ammonio-1,3-thiazol-4-yl)-2-[[(2-carboxypropan-2-yl)oxy]imino]acetyl]amino]-1-carboxypropan-2-yl]sulfamate | - |
? | |
cefotaxime + H2O | - |
Providencia stuartii | (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
ceftazidime + H2O | - |
Providencia stuartii | (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-{[(2-carboxypropan-2-yl)oxy]imino}acetyl]amino}(carboxy)methyl]-5-[(pyridin-1-ium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephaloridine + H2O | - |
Providencia stuartii | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
nitrocefin + H2O | - |
Providencia stuartii | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
penicillin G + H2O | - |
Providencia stuartii | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Synonyms | Comment | Organism |
---|---|---|
extended-spectrum beta-lactamase TEM-60 | - |
Providencia stuartii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
30 min, mutant enzyme R146S is stable, mutant enzyme K104E loses 50% of maximal activity, mutant enzyme P51L loses 95% of maximal activity, wild-type enzyme loses 80% of maximal activity | Providencia stuartii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
cefotaxime | mutant enzyme S164R | Providencia stuartii | |
0.45 | - |
(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | mutant enzyme S164R | Providencia stuartii | |
1.5 | - |
cefotaxime | wild-type enzyme | Providencia stuartii | |
1.5 | - |
(6R,7R)-3-[(acetyloxy)methyl]-7-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid | wild-type enzyme | Providencia stuartii | |
3.2 | - |
ampicillin | mutant enzyme P51L | Providencia stuartii | |
5 | - |
nitrocefin | wild-type enzyme | Providencia stuartii | |
8 | - |
nitrocefin | mutant enzyme P51L | Providencia stuartii | |
9 | - |
aztreonam | wild-type enzyme | Providencia stuartii | |
12 | - |
ceftazidime | wild-type enzyme | Providencia stuartii | |
15 | - |
cephaloridine | wild-type enzyme | Providencia stuartii | |
17 | - |
aztreonam | mutant enzyme P51L | Providencia stuartii | |
18 | - |
ampicillin | wild-type enzyme | Providencia stuartii | |
22 | - |
cephaloridine | mutant enzyme P51L | Providencia stuartii | |
23 | - |
penicillin G | mutant enzyme P51L | Providencia stuartii | |
26 | - |
penicillin G | wild-type enzyme | Providencia stuartii | |
33 | - |
penicillin G | mutant enzyme K104E | Providencia stuartii | |
60 | - |
ceftazidime | mutant enzyme P51L | Providencia stuartii | |
127 | - |
cephaloridine | mutant enzyme K104E | Providencia stuartii | |
146 | - |
ampicillin | mutant enzyme S164R | Providencia stuartii | |
150 | - |
aztreonam | mutant enzyme S164R | Providencia stuartii | |
176 | - |
nitrocefin | mutant enzyme K104E | Providencia stuartii | |
200 | - |
penicillin G | mutant enzyme S164R | Providencia stuartii | |
590 | - |
cephaloridine | mutant enzyme S164R | Providencia stuartii | |
600 | - |
nitrocefin | mutant enzyme S164R | Providencia stuartii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Providencia stuartii | isoelectric focusing, mutant enzyme K104E | - |
5.4 |
Providencia stuartii | isoelectric focusing, mutant enzyme P51L | - |
6 |
Providencia stuartii | isoelectric focusing, mutant enzyme S164R | - |
6.1 |