Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression in Escherichia coli | [Haemophilus] ducreyi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 18% inhibition at 3 mM | [Haemophilus] ducreyi | |
additional information | no inhibition by boric acid, cefotaxime, PMSF | [Haemophilus] ducreyi | |
potassium clavulanate | 96% inhibition at 0.005 mM | [Haemophilus] ducreyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
benzylpenicillin | pH 7.5, 37°C | [Haemophilus] ducreyi | |
0.05 | - |
nitrocefin | pH 7.5, 37°C | [Haemophilus] ducreyi | |
0.06 | - |
ampicillin | pH 7.5, 37°C | [Haemophilus] ducreyi | |
0.14 | - |
cefoperazone | pH 7.5, 37°C | [Haemophilus] ducreyi | |
0.26 | - |
carbencillin | pH 7.5, 37°C | [Haemophilus] ducreyi | |
0.45 | - |
cephalothin | pH 7.5, 37°C | [Haemophilus] ducreyi | |
1.4 | - |
cephaloridine | pH 7.5, 37°C | [Haemophilus] ducreyi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | [Haemophilus] ducreyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Haemophilus] ducreyi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli, 31.3fold by ammonium sulfate precipitation and Zn2+-affinity chromatography | [Haemophilus] ducreyi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2475 | - |
purified recombinant enzyme | [Haemophilus] ducreyi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
[Haemophilus] ducreyi | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
benzylpenicillin + H2O | best substrate | [Haemophilus] ducreyi | (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
carbenicillin + H2O | - |
[Haemophilus] ducreyi | (2R,4S)-2-{(R)-carboxy[2-carboxy(phenyl)acetamido]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefoperazone + H2O | - |
[Haemophilus] ducreyi | (2R)-2-[(R)-carboxy{[(2S)-2-[(4-ethyl-2,3-dioxopiperazine-1-carbonyl)amino]-2-(4-hydroxyphenyl)acetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | - |
[Haemophilus] ducreyi | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
[Haemophilus] ducreyi | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
[Haemophilus] ducreyi | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | [Haemophilus] ducreyi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | [Haemophilus] ducreyi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | [Haemophilus] ducreyi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
[Haemophilus] ducreyi | - |
- |
4.9 |