KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
cefamandole | beta-lactamase D | Staphylococcus aureus | |
0.0047 | - |
cephalothin | beta-lactamase D | Staphylococcus aureus | |
0.005 | - |
cefamandole | beta-lactamase A | Staphylococcus aureus | |
0.0052 | - |
cephapirin | beta-lactamase A | Staphylococcus aureus | |
0.0052 | - |
cephalothin | beta-lactamase C | Staphylococcus aureus | |
0.0059 | - |
nitrocefin | beta-lactamase D | Staphylococcus aureus | |
0.006 | - |
cefamandole | beta-lactamase B | Staphylococcus aureus | |
0.0062 | - |
cephalothin | beta-lactamase B | Staphylococcus aureus | |
0.0064 | - |
nitrocefin | beta-lactamase A | Staphylococcus aureus | |
0.0065 | - |
cefamandole | beta-lactamase C | Staphylococcus aureus | |
0.0068 | - |
cephalothin | beta-lactamase A | Staphylococcus aureus | |
0.0079 | - |
cephaloridine | beta-lactamase D | Staphylococcus aureus | |
0.011 | - |
cephaloridine | beta-lactamase A | Staphylococcus aureus | |
0.0115 | - |
cephaloridine | beta-lactamase C | Staphylococcus aureus | |
0.0122 | - |
cephapirin | beta-lactamase D | Staphylococcus aureus | |
0.0157 | - |
nitrocefin | beta-lactamase B | Staphylococcus aureus | |
0.018 | - |
cefazolin | beta-lactamase A | Staphylococcus aureus | |
0.0194 | - |
cephaloridine | beta-lactamase B | Staphylococcus aureus | |
0.0204 | - |
nitrocefin | beta-lactamase C | Staphylococcus aureus | |
0.0386 | - |
penicillin G | beta-lactamase B | Staphylococcus aureus | |
0.0472 | - |
cephapirin | beta-lactamase C | Staphylococcus aureus | |
0.0499 | - |
cefazolin | beta-lactamase D | Staphylococcus aureus | |
0.0511 | - |
penicillin G | beta-lactamase A | Staphylococcus aureus | |
0.0559 | - |
penicillin G | beta-lactamase C | Staphylococcus aureus | |
0.0795 | - |
cephapirin | beta-lactamase B | Staphylococcus aureus | |
0.1126 | - |
penicillin G | beta-lactamase D | Staphylococcus aureus | |
0.122 | - |
ampicillin | beta-lactamase C | Staphylococcus aureus | |
0.129 | - |
ampicillin | beta-lactamase D | Staphylococcus aureus | |
0.16 | - |
ampicillin | beta-lactamase B | Staphylococcus aureus | |
0.254 | - |
cefazolin | beta-lactamase B | Staphylococcus aureus | |
0.255 | - |
ampicillin | beta-lactamase A | Staphylococcus aureus | |
0.267 | - |
cefazolin | beta-lactamase C | Staphylococcus aureus | |
0.378 | - |
cefuroxime | beta-lactamase A | Staphylococcus aureus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Staphylococcus aureus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
SDS-PAGE | Staphylococcus aureus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
- |
- |
Staphylococcus aureus | - |
strains PC11, 22260, V137, FAR8, FAR10 | - |
Staphylococcus aureus PC1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
4 enzymes: beta-lactamase A-D | Staphylococcus aureus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
80 | - |
substrate penicillin G, beta-lactamase D | Staphylococcus aureus |
243 | - |
substrate penicillin G, beta-lactamase A | Staphylococcus aureus |
252 | - |
substrate penicillin G, beta-lactamase B | Staphylococcus aureus |
364 | - |
substrate penicillin G, beta-lactamase C | Staphylococcus aureus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ampicillin + H2O | - |
Staphylococcus aureus | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
ampicillin + H2O | - |
Staphylococcus aureus PC1 | (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? | |
cefamandole + H2O | - |
Staphylococcus aureus | (2R)-2-[(R)-carboxy{[(2R)-2-hydroxy-2-phenylacetyl]amino}methyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefazolin + H2O | - |
Staphylococcus aureus | (2R)-2-[(R)-carboxy[(1H-tetrazol-1-ylacetyl)amino]methyl]-5-[[(5-methyl-1,3,4-thiadiazol-2-yl)sulfanyl]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefuroxime + H2O | - |
Staphylococcus aureus | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cefuroxime + H2O | - |
Staphylococcus aureus PC1 | (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephaloridine + H2O | - |
Staphylococcus aureus | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephaloridine + H2O | - |
Staphylococcus aureus PC1 | (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate | - |
? | |
cephalothin + H2O | - |
Staphylococcus aureus | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephalothin + H2O | - |
Staphylococcus aureus PC1 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephapirin + H2O | - |
Staphylococcus aureus | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy{2-[(pyridin-4-yl)sulfanyl]acetamido}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
cephapirin + H2O | - |
Staphylococcus aureus PC1 | (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy{2-[(pyridin-4-yl)sulfanyl]acetamido}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
nitrocefin + H2O | - |
Staphylococcus aureus | (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid | - |
? | |
penicillin G + H2O | - |
Staphylococcus aureus | (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00667 | - |
cephalothin | beta-lactamase D | Staphylococcus aureus | |
0.00833 | - |
cefuroxime | beta-lactamase A | Staphylococcus aureus | |
0.0167 | - |
cephalothin | beta-lactamase A | Staphylococcus aureus | |
0.05 | - |
cefamandole | beta-lactamase A | Staphylococcus aureus | |
0.05 | - |
cefamandole | beta-lactamase D | Staphylococcus aureus | |
0.1 | - |
cephalothin | beta-lactamase B | Staphylococcus aureus | |
0.1 | - |
cephalothin | beta-lactamase C | Staphylococcus aureus | |
0.25 | - |
cephapirin | beta-lactamase D | Staphylococcus aureus | |
0.3 | - |
cefamandole | beta-lactamase C | Staphylococcus aureus | |
0.35 | - |
cefamandole | beta-lactamase B | Staphylococcus aureus | |
0.367 | - |
cephapirin | beta-lactamase A | Staphylococcus aureus | |
1 | - |
cefazolin | beta-lactamase A | Staphylococcus aureus | |
1.18 | - |
cephapirin | beta-lactamase C | Staphylococcus aureus | |
1.25 | - |
cephaloridine | beta-lactamase D | Staphylococcus aureus | |
1.82 | - |
cephapirin | beta-lactamase B | Staphylococcus aureus | |
1.92 | - |
cefazolin | beta-lactamase D | Staphylococcus aureus | |
1.95 | - |
cephaloridine | beta-lactamase A | Staphylococcus aureus | |
3.95 | - |
cefazolin | beta-lactamase C | Staphylococcus aureus | |
4.67 | - |
cefazolin | beta-lactamase B | Staphylococcus aureus | |
5.6 | - |
cephaloridine | beta-lactamase C | Staphylococcus aureus | |
6.65 | - |
cephaloridine | beta-lactamase B | Staphylococcus aureus | |
13.7 | - |
nitrocefin | beta-lactamase C | Staphylococcus aureus | |
16.5 | - |
nitrocefin | beta-lactamase B | Staphylococcus aureus | |
23.2 | - |
nitrocefin | beta-lactamase A | Staphylococcus aureus | |
25.6 | - |
nitrocefin | beta-lactamase D | Staphylococcus aureus | |
46 | - |
penicillin G | beta-lactamase D | Staphylococcus aureus | |
133 | - |
ampicillin | beta-lactamase D | Staphylococcus aureus | |
156 | - |
penicillin G | beta-lactamase B | Staphylococcus aureus | |
171 | - |
penicillin G | beta-lactamase A | Staphylococcus aureus | |
210 | - |
penicillin G | beta-lactamase C | Staphylococcus aureus | |
308 | - |
ampicillin | beta-lactamase A | Staphylococcus aureus | |
355 | - |
ampicillin | beta-lactamase C | Staphylococcus aureus | |
412 | - |
ampicillin | beta-lactamase B | Staphylococcus aureus |