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Literature summary for 3.5.2.6 extracted from

  • Crowder, M.W.; Walsh, T.R.; Banovic, L.; Pettit, M.; Spencer, J.
    Overexpression, purification, and characterization of the cloned metallo-beta-lactamase L1 from Stenotrophomonas maltophilia (1998), Antimicrob. Agents Chemother., 42, 921-926.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 cefoxitin
-
 Stenotrophomonas maltophilia
0.004
-
 cefmetazole
-
 Stenotrophomonas maltophilia
0.005
-
 moxalactam
-
 Stenotrophomonas maltophilia
0.008
-
 ceftizoxime
-
 Stenotrophomonas maltophilia
0.012
-
 nitrocefin
-
 Stenotrophomonas maltophilia
0.022
-
 clavulanic acid
-
 Stenotrophomonas maltophilia
0.03
-
 cefsulodin
-
 Stenotrophomonas maltophilia
0.031
-
 cefaclor
-
 Stenotrophomonas maltophilia
0.043
-
 cephalothin
-
 Stenotrophomonas maltophilia
0.06
-
 azlocillin
-
 Stenotrophomonas maltophilia
0.075
-
 penicillin G
-
 Stenotrophomonas maltophilia
0.075
-
 biapenem
-
 Stenotrophomonas maltophilia
0.1
-
 cefprozil
-
 Stenotrophomonas maltophilia
0.13
-
 cefuroxime
-
 Stenotrophomonas maltophilia
0.13
-
 cefepime
-
 Stenotrophomonas maltophilia
0.16
-
 cefotaxime
-
 Stenotrophomonas maltophilia
0.17
-
 cephaloridine
-
 Stenotrophomonas maltophilia
0.18
-
 cephalosporin C
-
 Stenotrophomonas maltophilia
0.2
-
 piperacillin
-
 Stenotrophomonas maltophilia
0.25
-
 cefadroxil
-
 Stenotrophomonas maltophilia
0.26
-
 cephradine
-
 Stenotrophomonas maltophilia
0.3
-
 ampicillin
-
 Stenotrophomonas maltophilia
0.49
-
 ticarcillin
-
 Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ required for activity Stenotrophomonas maltophilia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28800
-
 gel filtration Stenotrophomonas maltophilia

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ampicillin + H2O
-
 Stenotrophomonas maltophilia (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
azlocillin + H2O
-
 Stenotrophomonas maltophilia (2R,4S)-2-[(R)-carboxy({(2R)-2-[(2-oxoimidazolidine-1-carbonyl)amino]-2-phenylacetyl}amino)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
biapenem + H2O
-
 Stenotrophomonas maltophilia (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
cefaclor + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-5-chloro-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefadroxil + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefepime + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-[[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-5-[(1-methylpyrrolidinium-1-yl)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cefmetazole + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(S)-carboxy{2-[(cyanomethyl)sulfanyl]acetamido}methoxymethyl]-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefotaxime + H2O
-
 Stenotrophomonas maltophilia (2R)-5-[(acetyloxy)methyl]-2-[(R)-[[(2E)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino](carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefoxitin + H2O
-
 Stenotrophomonas maltophilia (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefprozil + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-{[(2R)-2-amino-2-(4-hydroxyphenyl)acetyl]amino}(carboxy)methyl]-5-[(1E)-prop-1-en-1-yl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefsulodin + H2O
-
 Stenotrophomonas maltophilia (2R)-5-[(4-carbamoylpyridin-1-ium-1-yl)methyl]-2-[(R)-carboxy{[(2R)-2-phenyl-2-sulfoacetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
ceftizoxime + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-{[(2Z)-2-(2-amino-1,3-thiazol-4-yl)-2-(methoxyimino)acetyl]amino}(carboxy)methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cefuroxime + H2O
-
 Stenotrophomonas maltophilia (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephaloridine + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
cephalosporin C + H2O
-
 Stenotrophomonas maltophilia N6-[(R)-{(2R)-5-[(acetyloxy)methyl]-4-carboxy-3,6-dihydro-2H-1,3-thiazin-2-yl}(carboxy)methyl]-6-oxo-D-lysine
-
 ?
cephalothin + H2O
-
 Stenotrophomonas maltophilia (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
cephradine + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-{[(2R)-2-amino-2-(cyclohexa-1,4-dien-1-yl)acetyl]amino}(carboxy)methyl]-5-methyl-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
clavulanic acid + H2O
-
 Stenotrophomonas maltophilia (2R,4R,5Z)-2-(carboxymethyl)-5-(2-hydroxyethylidene)-1,3-oxazolidine-4-carboxylic acid
-
 ?
moxalactam + H2O
-
 Stenotrophomonas maltophilia (2R)-2-{(R)-carboxy[2-carboxy(4-hydroxyphenyl)acetamido]methoxymethyl}-5-{[(1-methyl-1H-tetrazol-5-yl)sulfanyl]methyl}-3,6-dihydro-2H-1,3-oxazine-4-carboxylic acid
-
 ?
nitrocefin + H2O
-
 Stenotrophomonas maltophilia (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
penicillin G + H2O
-
 Stenotrophomonas maltophilia (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
piperacillin + H2O
-
 Stenotrophomonas maltophilia (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
ticarcillin + H2O
-
 Stenotrophomonas maltophilia (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Subunits

Subunits Comment Organism
tetramer 4* 28800, gel filtration Stenotrophomonas maltophilia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0833
-
 cefsulodin
-
 Stenotrophomonas maltophilia
0.15
-
 moxalactam
-
 Stenotrophomonas maltophilia
0.333
-
 cefepime
-
 Stenotrophomonas maltophilia
2.2
-
 cefoxitin
-
 Stenotrophomonas maltophilia
2.7
-
 ceftizoxime
-
 Stenotrophomonas maltophilia
4.7
-
 cefmetazole
-
 Stenotrophomonas maltophilia
11
-
 clavulanic acid
-
 Stenotrophomonas maltophilia
15
-
 cefprozil
-
 Stenotrophomonas maltophilia
16
-
 cephradine
-
 Stenotrophomonas maltophilia
23
-
 cefadroxil
-
 Stenotrophomonas maltophilia
31
-
 nitrocefin
-
 Stenotrophomonas maltophilia
37
-
 azlocillin
-
 Stenotrophomonas maltophilia
53
-
 cefuroxime
-
 Stenotrophomonas maltophilia
60
-
 cefaclor
-
 Stenotrophomonas maltophilia
64
-
 biapenem
-
 Stenotrophomonas maltophilia
65
-
 cephalothin
-
 Stenotrophomonas maltophilia
67
-
 cephaloridine
-
 Stenotrophomonas maltophilia
140
-
 cefotaxime
-
 Stenotrophomonas maltophilia
280
-
 cephalosporin C
-
 Stenotrophomonas maltophilia
410
-
 penicillin G
-
 Stenotrophomonas maltophilia
420
-
 piperacillin
-
 Stenotrophomonas maltophilia
440
-
 ticarcillin
-
 Stenotrophomonas maltophilia
580
-
 ampicillin
-
 Stenotrophomonas maltophilia