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Literature summary for 3.5.2.3 extracted from

  • Wang, C.C.; Tsau, H.W.; Chen, W.T.; Huang, C.Y.
    Identification and characterization of a putative dihydroorotase, KPN01074, from Klebsiella pneumoniae (2010), Protein J., 29, 445-452.
    View publication on PubMed
Search for more literature for 3.5.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
D251A complete loss of activity Klebsiella pneumoniae
D251E 4fold increase in activity Klebsiella pneumoniae
H140A complete loss of activity Klebsiella pneumoniae
H178A complete loss of activity Klebsiella pneumoniae
H17A complete loss of activity Klebsiella pneumoniae
H19A complete loss of activity Klebsiella pneumoniae
L103A complete loss of activity Klebsiella pneumoniae

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Klebsiella pneumoniae
Co2+
-
 Klebsiella pneumoniae
Mn2+
-
 Klebsiella pneumoniae
Ni2+
-
 Klebsiella pneumoniae
phosphate
-
 Klebsiella pneumoniae
Zn2+
-
 Klebsiella pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 (S)-dihydroorotate pH 9.0, 25°C Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, calculated Klebsiella pneumoniae
80000
-
 gel filtration Klebsiella pneumoniae

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae A6T7D6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.14
-
 no addition of metal ions to culture medium, pH 9.0, 25°C Klebsiella pneumoniae
10.57
-
 presence of Mn2+ in culture medium, pH 9.0, 25°C Klebsiella pneumoniae
14.46
-
 presence of Ni2+ in culture medium, pH 9.0, 25°C Klebsiella pneumoniae
22.22
-
 presence of Mg2+ in culture medium, pH 9.0, 25°C Klebsiella pneumoniae
22.79
-
 presence of Co2+ in culture medium, pH 9.0, 25°C Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + H2O
-
 Klebsiella pneumoniae N-carbamoyl-L-aspartate
-
 ?
additional information no substrate: hydantoin, dihydrouracil, phthalimide, and 3-imonoisoindolinone Klebsiella pneumoniae ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, calculated Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Klebsiella pneumoniae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life above 150 min Klebsiella pneumoniae
65
-
 half-life 30 min Klebsiella pneumoniae
70
-
 half-life 8 min Klebsiella pneumoniae

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 stability decreases in the order pH 8 pH pH 6 pH 9 Klebsiella pneumoniae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.15
-
 pH 9.0, 25°C Klebsiella pneumoniae Co2+
0.16
-
 pH 9.0, 25°C Klebsiella pneumoniae Cd2+
0.19
-
 pH 9.0, 25°C Klebsiella pneumoniae Zn2+
0.24
-
 pH 9.0, 25°C Klebsiella pneumoniae Mn2+
3.29
-
 pH 9.0, 25°C Klebsiella pneumoniae Ni2+
200
-
 pH 9.0, 25°C Klebsiella pneumoniae phosphate