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Literature summary for 3.5.2.3 extracted from

  • Krungkrai, S.R.; Wutipraditkul, N.; Krungkrai, J.
    Dihydroorotase of human malarial parasite Plasmodium falciparum differs from host enzyme (2008), Biochem. Biophys. Res. Commun., 366, 821-826.
    View publication on PubMed
Search for more literature for 3.5.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
5-aminoorotate
-
 Plasmodium falciparum
5-bromoorotate
-
 Plasmodium falciparum
5-Fluoroorotate
-
 Plasmodium falciparum
5-iodoorotate
-
 Plasmodium falciparum
5-Methylorotate
-
 Plasmodium falciparum
Co2+
-
 Plasmodium falciparum
diethyl dicarbonate strong inhibition at 1 mM, activity is restored more than 95% when the enzyme is preincubated with both 1 mM diethyl dicarbonate and 5 mM L-carbamoyl-L-aspartate Plasmodium falciparum
additional information EDTA and 1,10-phenanthroline have no effect on the enzyme activity; L-carbamoyl-L-aspartate and L-dihydroorotate have no inhibitory effect on the enzyme at the pH 7.4 Plasmodium falciparum
Orotate
-
 Plasmodium falciparum
Zn2+ complete inhibition Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 L-dihydroorotate at pH 6.0 Plasmodium falciparum
0.285
-
 N-(aminocarbonyl)-L-aspartic acid at pH 9.0 Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 gel filtration Plasmodium falciparum
41720
-
 calculated from amino acid sequence Plasmodium falciparum
42000
-
 SDS-PAGE Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0014
-
 crude extract Plasmodium falciparum
0.4985
-
 after 356fold purification Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-dihydroorotate + H2O
-
 Plasmodium falciparum N-carbamoyl-L-aspartate
-
 r

Subunits

Subunits Comment Organism
monomer 1 * 40000, gel filtration, 75% of the total activity is associated with the monomeric form Plasmodium falciparum

Synonyms

Synonyms Comment Organism
DHOase the malarial enzyme shares characteristics of both type I and type II DHOases Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.073
-
 N-(aminocarbonyl)-L-aspartic acid at pH 9.0 Plasmodium falciparum
1.492
-
 L-dihydroorotate at pH 6.0 Plasmodium falciparum

pH Range

pH Minimum pH Maximum Comment Organism
4 11
-
 Plasmodium falciparum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.07
-
 5-Fluoroorotate for the ring cleavage reaction Plasmodium falciparum
0.145
-
 5-Fluoroorotate for the ring cyclization reaction Plasmodium falciparum
0.16
-
 5-aminoorotate for the ring cleavage reaction Plasmodium falciparum
0.302
-
 5-Methylorotate for the ring cleavage reaction Plasmodium falciparum
0.44
-
 5-aminoorotate for the ring cyclization reaction Plasmodium falciparum
0.56
-
 5-Methylorotate for the ring cyclization reaction Plasmodium falciparum
0.66
-
 Orotate for the ring cyclization reaction Plasmodium falciparum
0.95
-
 Orotate for the ring cleavage reaction Plasmodium falciparum
1.46
-
 5-bromoorotate for the ring cyclization reaction Plasmodium falciparum
2.6
-
 5-bromoorotate for the ring cleavage reaction Plasmodium falciparum
2.85
-
 Zn2+
-
 Plasmodium falciparum
3.5
-
 5-iodoorotate Ki above 3.5 mM, for the ring cleavage reaction Plasmodium falciparum
3.5
-
 5-iodoorotate Ki above 3.5 mM, for the ring cyclization reaction Plasmodium falciparum
3.55
-
 Co2+
-
 Plasmodium falciparum

pI Value

Organism Comment pI Value Maximum pI Value
Plasmodium falciparum calculated from amino acid sequence
-
 7.45