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Literature summary for 3.5.2.3 extracted from

  • Robles Lopez, S.M.; Hortua Triana, M.A.; Zimmermann, B.H.
    Cloning and preliminary characterization of the dihydroorotase from Toxoplasma gondii (2006), Mol. Biochem. Parasitol., 148, 93-98.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain X7014 Toxoplasma gondii
expression in Escherichia coli mutant strain SØ1263 and strain X7014 Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
dihydroorotate purified recombinant enzyme, at 37°C Toxoplasma gondii
0.0643
-
L-dihydroorotate purified recombinant protein Toxoplasma gondii
0.323
-
N-carbamoyl-L-aspartate purified recombinant protein Toxoplasma gondii
0.323
-
N-(aminocarbonyl)-L-aspartic acid purified recombinant enzyme, at 37°C Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
Toxoplasma gondii
Zn2+ contains 3.33 mol zinc per mol protein Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44200
-
estimated from sequence of cDNA Toxoplasma gondii
45600
-
gel filtration, laser light scattering Toxoplasma gondii
45600
-
gel filtration and laser light scattering Toxoplasma gondii

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana O04904
-
-
Escherichia coli P05020
-
-
Homo sapiens P27708
-
-
Mesocricetus auratus P08955
-
-
Mycobacterium leprae
-
-
-
Plasmodium berghei A0A509AX79
-
-
Saccharomyces cerevisiae
-
-
-
Toxoplasma gondii Q8MXA5 strain RH lambda Zap II cDNA library, strain PC117 and 5TN
-
Toxoplasma gondii Q8MXA5 strains RH, PC117, and 5TN
-
Trypanosoma cruzi O76138
-
-
Ustilago maydis P31301
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sephacel column chromatography Toxoplasma gondii
recombinant protein purified to homogeneity Toxoplasma gondii

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
Toxoplasma gondii
-
tachyzoite maintained by serial passage in the peritoneal cavity of CFW mice Toxoplasma gondii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.3
-
in the biosynthetic direction Toxoplasma gondii
18.33
-
purified recombinant enzyme Toxoplasma gondii
18.4
-
in the degradative direction Toxoplasma gondii

Storage Stability

Storage Stability Organism
-70°C, HEPES buffer, pH 7.4, 0.5% glycerol Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroorotate + H2O
-
Toxoplasma gondii N-carbamoyl-L-aspartate
-
r
L-dihydroorotate + H2O
-
Toxoplasma gondii N-carbamoyl-L-aspartate
-
r
N-carbamoyl-L-aspartate
-
Toxoplasma gondii L-dihydroorotate + H2O
-
r

Subunits

Subunits Comment Organism
monomer 1 * 44200, SDS-PAGE Toxoplasma gondii
monomer 1 * 45600, gel filtration and laser light scattering Toxoplasma gondii

Synonyms

Synonyms Comment Organism
DHOase
-
Saccharomyces cerevisiae
DHOase
-
Mycobacterium leprae
DHOase
-
Homo sapiens
DHOase
-
Mesocricetus auratus
DHOase
-
Toxoplasma gondii
DHOase
-
Plasmodium berghei
DHOase
-
Ustilago maydis
DHOase
-
Arabidopsis thaliana
DHOase
-
Escherichia coli
DHOase
-
Trypanosoma cruzi

pH Range

pH Minimum pH Maximum Comment Organism
5 9 in the biosynthetic direction Toxoplasma gondii
5 7.8 in the degradative direction Toxoplasma gondii

pI Value

Organism Comment pI Value Maximum pI Value
Toxoplasma gondii isoelectric focusing
-
5.72