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Literature summary for 3.5.2.3 extracted from
- Mechanism of the dihydroorotase reaction (2004), Biochemistry, 43, 16285-16292.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli strain X7014a | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| x-ray structure | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D250A | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
| D250E | reduced catalytic activity compared to wild type | Escherichia coli |
| D250H | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
| D250N | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
| D250S | reduced catalytic activity compared to wild type | Escherichia coli |
| H254N | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
| N44A | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
| R20K | reduced catalytic activity compared to wild type | Escherichia coli |
| R20M | catalytically inactive | Escherichia coli |
| R20Q | less than 1% of the activity possessed by the wild type enzyme | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
carbamoyl aspartate | mutants D250A, D250H, D250N, R20Q, R20M, N44A, H254N inactive | Escherichia coli |  |
| 0.009 | - |
thiodihydroorotate | Cd/Cd-dihydroorotase, pH 8.0 | Escherichia coli | |
| 0.03 | - |
thiodihydroorotate | Zn/Zn-dihydroorotase, pH 8.0 | Escherichia coli | |
| 0.08 | - |
dihydroorotate | Zn/Zn-dihydroorotase, pH 8.0 | Escherichia coli | |
| 0.23 | - |
dihydroorotate | Cd/Cd-dihydroorotase, pH 8.0 | Escherichia coli | |
| 0.51 | - |
carbamoyl aspartate | mutant D250S | Escherichia coli | |
| 0.7 | - |
dihydroorotate | Co/Co-dihydroorotase, pH 8.0 | Escherichia coli | |
| 0.9 | - |
carbamoyl aspartate | mutant R20K | Escherichia coli | |
| 1.7 | - |
carbamoyl aspartate | wild type, Zn/Zn-dihydroorotase, pH 5.8 | Escherichia coli | |
| 1.9 | - |
carbamoyl aspartate | mutant D250E | Escherichia coli | |
| 4 | - |
carbamoyl aspartate | Cd/Cd-dihydroorotase, pH 5.8 | Escherichia coli | |
| 15 | - |
carbamoyl aspartate | Co/Co-dihydroorotase, pH 5.8 | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Escherichia coli | |
| Co2+ | - |
Escherichia coli | |
| Zn2+ | activity of Zn2+ > Co2+ > Cd2+ | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P05020 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| chromatography, gel filtration, resource-Q anion exchange column | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, Bis-Tris propane buffer, pH 7.0, 20% glycerol | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
| dihydroorotate + H2O | - |
Escherichia coli | N-carbamoyl-L-aspartate | - |
r | |
| N-carbamoyl-L-aspartate | - |
Escherichia coli | (S)-dihydroorotate + H2O | - |
r | |
| thio-dihydroorotate + H2O | - |
Escherichia coli | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DHO | - |
Escherichia coli |
| pyrC | - |
Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.9 | synthesis reaction | Escherichia coli |
| 7.8 | 8.5 | hydrolysis reaction | Escherichia coli |
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