Literature summary for 3.5.2.3 extracted from

Williams, N.K.; Manthey, M.K.; Hambley, T.W.; O'Donoghue, S.I.; Keegan, M.; Chapman, B.E.; Christopherson, R.I.
Catalysis by hamster dihydroorotase: zinc binding, site-directed mutagenesis, and interaction with inhibitors (1995), Biochemistry, 34, 11344-11352.

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Inhibitors

Inhibitors	Comment	Organism	Structure
2-oxo-1,2,3,6-tetrahydropyrimidine-4,6-dicarboxylate	-	Cricetinae
cis-2-oxohexahydropyrimidine-4,6-dicarboxylate	-	Cricetinae
L-6-thiodihydroorotate	-	Cricetinae
trans-2-oxohexahydropyrimidine-4,6-dicarboxylate	-	Cricetinae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	L-dihydroorotate	wild type (pure) and E301A	Cricetinae
0.007	-	L-dihydroorotate	wild type	Cricetinae
0.011	-	L-dihydroorotate	H186A (pure)	Cricetinae
0.016	-	L-dihydroorotate	K23R (pure)	Cricetinae
0.099	-	L-dihydroorotate	D230E	Cricetinae
0.45	-	L-dihydroorotate	K239G (pure)	Cricetinae

Organism

Organism	UniProt	Comment	Textmining
Cricetinae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
from overexpressing Escherichia coli	Cricetinae

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Release 2023.1 (January 2023)