Literature summary for 3.5.2.3 extracted from

Krungkrai, J.; Cerami, A.; Henderson, G.B.
Pyrimidine biosynthesis in parasitic protozoa: purification of a monofunctional dihydroorotase from Plasmodium berghei and Crithidia fasciculata (1990), Biochemistry, 29, 6270-6275.

Search for more literature for 3.5.2.3

Activating Compound

Activating Compound	Comment	Organism	Structure
Dimethylsulfoxide	increases the catalytic efficiency on the ring cyclization reaction, but not on the ring hydrolysis	Crithidia fasciculata
Dimethylsulfoxide	increases the catalytic efficiency on the ring cyclization reaction, but not on the ring hydrolysis	Plasmodium berghei

Inhibitors

Inhibitors	Comment	Organism	Structure
diethyldicarbonate	-	Crithidia fasciculata
diethyldicarbonate	-	Plasmodium berghei

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.026	-	L-dihydroorotate	-	Crithidia fasciculata
0.026	-	L-dihydroorotate	-	Plasmodium berghei
0.846	-	L-carbamoylaspartate	-	Crithidia fasciculata
0.846	-	L-carbamoylaspartate	-	Plasmodium berghei

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	1 * 38000, SDS-PAGE	Crithidia fasciculata
38000	-	1 * 38000, SDS-PAGE	Plasmodium berghei
40000	-	gel filtration	Plasmodium berghei
42000	-	gel filtration	Crithidia fasciculata
44000	-	1 * 44000, SDS-PAGE	Crithidia fasciculata

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
N-carbamoyl-L-aspartate	Crithidia fasciculata	-	L-dihydroorotate + H2O	-	r
N-carbamoyl-L-aspartate	Plasmodium berghei	-	L-dihydroorotate + H2O	-	r

Organism

Organism	UniProt	Comment	Textmining
Crithidia fasciculata	-	-	-
Plasmodium berghei	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Crithidia fasciculata
-	Plasmodium berghei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-carbamoylaspartate	-	Crithidia fasciculata	L-dihydroorotate + H2O	-	r
L-carbamoylaspartate	-	Plasmodium berghei	L-dihydroorotate + H2O	-	r
N-carbamoyl-L-aspartate	-	Crithidia fasciculata	L-dihydroorotate + H2O	-	r
N-carbamoyl-L-aspartate	-	Plasmodium berghei	L-dihydroorotate + H2O	-	r

Subunits

Subunits	Comment	Organism
monomer	1 * 38000, SDS-PAGE	Crithidia fasciculata
monomer	1 * 38000, SDS-PAGE	Plasmodium berghei
monomer	1 * 44000, SDS-PAGE	Crithidia fasciculata

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.183	-	L-dihydroorotate	-	Plasmodium berghei
0.653	-	L-carbamoylaspartate	-	Crithidia fasciculata
0.653	-	L-carbamoylaspartate	-	Plasmodium berghei
4.31	-	L-dihydroorotate	-	Crithidia fasciculata
4.31	-	L-dihydroorotate	-	Plasmodium berghei

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Release 2023.1 (January 2023)