Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli | Glycine max |
Crystallization (Comment) | Organism |
---|---|
- |
Glycine max |
Protein Variants | Comment | Organism |
---|---|---|
E199A | site-directed mutagenesis, mutant devoid of detectable catalytic activity | Glycine max |
E408A | site-directed mutagenesis, mutant devoid of detectable catalytic activity | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5-hydroxyisourate | pH 7.5, 37°C, recombinant enzyme lacking the signal peptide | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Glycine max | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63730 | - |
calculated from cDNA sequence | Glycine max |
68000 | - |
SDS-PAGE, gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyisourate + H2O | Glycine max | - |
5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Glycine max | Q8S3J3 | soybean, cv Williams | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyisourate + H2O | - |
Glycine max | 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HIUHase | - |
Glycine max |
hydroxyisourate hydrolase | - |
Glycine max |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
24 | - |
5-hydroxyisourate | pH 7.5, 37°C, recombinant enzyme lacking the signal peptide | Glycine max |