Cloned (Comment) | Organism |
---|---|
gene HOS2, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-atgegd enzyme in Spodoptera frugiperda Sf9 cells | Candida albicans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
MGCD 290 | an enzyme Hos2 inhibitor, for use in combination with azoles, such as fluconazole, for fungal infections | Candida albicans | |
MS-275 | 56.3% inhibition at 0.01 mM, 46.3% inhibition at 0.001 mM | Candida albicans | |
suberoylanilide hydroxamic acid | - |
Candida albicans | |
trichostatin A | - |
Candida albicans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Candida albicans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-lysine-histone + H2O | Candida albicans | - |
acetate + histone | - |
? | |
N-acetyl-lysine-histone + H2O | Candida albicans ATCC 90028 | - |
acetate + histone | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida albicans | - |
gene HOS2 | - |
Candida albicans ATCC 90028 | - |
gene HOS2 | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-atgegd enzyme from Spodoptera frugiperda Sf9 cells by nickel affinity chromatography and dialysis | Candida albicans |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Candida albicans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-acetyl-Lys-7-amido-4-methylcoumarin + H2O | - |
Candida albicans | Boc-acetyl-Lys + 7-amino-4-methylcoumarin | - |
? | |
Boc-acetyl-Lys-7-amido-4-methylcoumarin + H2O | - |
Candida albicans ATCC 90028 | Boc-acetyl-Lys + 7-amino-4-methylcoumarin | - |
? | |
additional information | purified fungal Hos2 protein consistently deacetylates tubulins, rather than histones from trichostatin A-treated cells. Hos2 does not show any sirtuin activity, the enzyme deacetylates human nuclear histones from HeLa cells and human acetylated tubulin from Jurkat cells, overview | Candida albicans | ? | - |
? | |
additional information | purified fungal Hos2 protein consistently deacetylates tubulins, rather than histones from trichostatin A-treated cells. Hos2 does not show any sirtuin activity, the enzyme deacetylates human nuclear histones from HeLa cells and human acetylated tubulin from Jurkat cells, overview | Candida albicans ATCC 90028 | ? | - |
? | |
N-acetyl-lysine-histone + H2O | - |
Candida albicans | acetate + histone | - |
? | |
N-acetyl-lysine-histone + H2O | - |
Candida albicans ATCC 90028 | acetate + histone | - |
? |
Synonyms | Comment | Organism |
---|---|---|
class I HDAC | - |
Candida albicans |
HDAC | - |
Candida albicans |
Hos2 | - |
Candida albicans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Candida albicans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Candida albicans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000028 | - |
pH 8.0, 37°C, recombinant enzyme | Candida albicans | trichostatin A | |
0.000065 | - |
pH 8.0, 37°C, recombinant enzyme | Candida albicans | suberoylanilide hydroxamic acid |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of Hos2, the catalytic subunit of the Set3 complex, produces a phenotype resembling inhibition of the Set3C by trichostatin-A | Candida albicans |
additional information | enzyme Hos2 is not inhibited by class I inhibitors such as MS-275 | Candida albicans |
physiological function | the Hos2/Set3 histone deacetylase complex (Set3C) plays a key role in the conversion of white phase to virulent opaque phase in Candida albicans. Azole resistance can be reversed by the co-administration of a histone deacetylase inhibitor, suggesting that resistance is mediated by epigenetic mechanisms possibly involving the fungal deacetylase Hos2 | Candida albicans |