Literature summary for 3.5.1.98 extracted from

Zou, H.; Wu, Y.; Navre, M.; Sang, B.C.
Characterization of the two catalytic domains in histone deacetylase 6 (2006), Biochem. Biophys. Res. Commun., 341, 45-50.

Search for more literature for 3.5.1.98

Cloned(Commentary)

Cloned (Comment)	Organism
expression in baculoviral system, His-tagged protein	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
H216A	mutation in the first catalytic domain of enzyme, slight decrease in catalytic rate	Homo sapiens
H611A	mutation in second catalytic domain of enzyme, more than 5000fold decrease in catalytic rate	Homo sapiens
additional information	enzyme contains two potentially functional catalytic domains. Expression of first domain alone does not result in catalytic activity. The catalytic domain II alone shows only a modest decrease in substrate binding and product formation rate compared to wild-type	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.000015	-	Fluor de Lys	mt H611A, pH 7.2, 23°C	Homo sapiens
0.0024	-	Fluor de Lys	catalytic domain II, pH 7.2, 23°C	Homo sapiens
0.0033	-	Fluor de Lys	wild-type, pH 7.2, 23°C	Homo sapiens
0.0038	-	Fluor de Lys	mutant H216A, pH 7.2, 23°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9UBN7	isoform HDAC6, contains two potentially functional catalytic domains	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Fluor de Lys + H2O	-	Homo sapiens	?	-	?
RHK(acetyl)K(acetyl)-fluorophore + H2O	i.e. Fluor de Lys, fluorogenic substrate	Homo sapiens	RHKK-fluorophore + acetate	-	?

Synonyms

Synonyms	Comment	Organism
HDAC6	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0019	-	Fluor de Lys	mt H611A, pH 7.2, 23°C	Homo sapiens
0.037	-	Fluor de Lys	catalytic domain II, pH 7.2, 23°C	Homo sapiens
0.27	-	Fluor de Lys	mutant H216A, pH 7.2, 23°C	Homo sapiens
0.29	-	Fluor de Lys	wild-type, pH 7.2, 23°C	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)