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Literature summary for 3.5.1.70 extracted from

  • Hormigo, D.; de la Mata, I.; Acebal, C.; Arroyo, M.
    Immobilized aculeacin A acylase from Actinoplanes utahensis: characterization of a novel biocatalyst (2010), Biores. Technol., 101, 4261-4268.
    View publication on PubMed
Search for more literature for 3.5.1.70

Application

Application Comment Organism
biotechnology recombinant enzyme covalently immobilized onto several epoxy-activated supports in order to obtain a robust biocatalyst to be used in industrial bioreactors. The best biocatalyst is obtained by attaching the enzyme on Sepabeads EC-EP5 Actinoplanes utahensis

Cloned(Commentary)

Cloned (Comment) Organism
recombinant enzyme covalently immobilized onto several epoxy-activated supports in order to obtain a robust biocatalyst to be used in industrial bioreactors. The best biocatalyst is obtained by attaching the enzyme on Sepabeads EC-EP5 Actinoplanes utahensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 penicillin K recombinant immobilized enzyme, Vmax: 7.6 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C Actinoplanes utahensis
3.9
-
 penicillin dihydroF recombinant immobilized enzyme, Vmax: 1.45 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C Actinoplanes utahensis
6.3
-
 aculeacin A recombinant immobilized enzyme, Vmax: 35.2 micromol of hydrolzed aculeacin A min-1/g wet biocatalyst, pH 7, 60°C Actinoplanes utahensis
6.45
-
 Penicillin V recombinant immobilized enzyme, Vmax: 35.2 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C Actinoplanes utahensis
9.5
-
 penicillin F recombinant immobilized enzyme, Vmax: 0.54 micromol of produced 6-aminopenicillanic acid min-1/g wet biocatalyst, pH 8, 45°C Actinoplanes utahensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
aculeacin A + H2O Actinoplanes utahensis
-
 ? + palmitic acid
-
 ?

Organism

Organism UniProt Comment Textmining
Actinoplanes utahensis
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 35.2 U/g wet carrier recombinant immobilized enzyme, hydrolysis of penicillin V, pH 8, 45°C Actinoplanes utahensis

Storage Stability

Storage Stability Organism
the recombinant immobilized enzyme can be recycled for at least 30 consecutive batch reactions at pH 8.0 and 45°C without loss of catalytic activity. Both soluble and immobilized enzyme apparently suffers no loss of enzymatic activity when stored at 45°C for at least 550 min Actinoplanes utahensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aculeacin A + H2O
-
 Actinoplanes utahensis ? + palmitic acid
-
 ?
additional information enzyme hydrolyzes the acyl moieties of antifungal echinocandin antibiotics, efficiently hydrolyzes penicillin V and natural aliphatic penicillins to yield 6-aminopenicillanic acid Actinoplanes utahensis ?
-
 ?
penicillin dihydroF + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + hexanoic acid
-
 ?
penicillin F + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + (3E)-hex-3-enoic acid
-
 ?
penicillin K + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + octanoic acid
-
 ?
penicillin V + H2O
-
 Actinoplanes utahensis 6-aminopenicillanic acid + phenoxyacetic acid
-
 ?

Synonyms

Synonyms Comment Organism
aculeacin A acylase
-
 Actinoplanes utahensis
AuAAC
-
 Actinoplanes utahensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
 assay at Actinoplanes utahensis
60
-
 assay at, substrate aculeacin A Actinoplanes utahensis
75
-
 recombinant immobilized enzyme, highest activity with penicillin V, above the activity decreased very quickly as a result of the denaturation of the enzyme, pH 8.0 Actinoplanes utahensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 half-life of the soluble enzyme at 65°C is estimate, t1/2: 477 min Actinoplanes utahensis
45
-
 both soluble and immobilized enzyme apparently suffers no loss of enzymatic activity when stored at 45°C for at least 550 min Actinoplanes utahensis
65
-
 the recombinant immobilized enzyme is highly thermostable since it suffers no loss of activity at 65°C and pH 8.0 during 3 h Actinoplanes utahensis
75
-
 the recombinant immobilized enzyme is completely deactivated after its incubation during two hours at temperatures above 75°C Actinoplanes utahensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, substrate aculeacin A Actinoplanes utahensis
8
-
 assay at Actinoplanes utahensis
8.5
-
 recombinant immobilized enzyme, highest activity with penicillin V, above the activity decreases very quickly as a result of the denaturation of the enzyme, 40°C Actinoplanes utahensis

pH Range

pH Minimum pH Maximum Comment Organism
6.5 11 ca. 17 U/g wet carrier at pH 6.5 and ca. 20 U/g wet carrier at pH 11 Actinoplanes utahensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 11 the immobilized enzyme is stable at the whole pH range from pH 6.5 to 11. Stability is tested for the storage of the biocatalyst for two hours at different pH values at 4°C Actinoplanes utahensis

General Information

General Information Comment Organism
physiological function enzyme hydrolyzes the acyl moieties of antifungal echinocandin antibiotics, efficiently hydrolyzes penicillin V and natural aliphatic penicillins to yield 6-aminopenicillanic acid Actinoplanes utahensis