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Literature summary for 3.5.1.38 extracted from

  • Elzainy, T.A.; Ali, T.H.
    Detection of the antitumor glutaminase-asparaginase in the filamentous fungi (2006), J. Appl. Sci., 6, 1389-1395.
No PubMed abstract available
Search for more literature for 3.5.1.38

Activating Compound

Activating Compound Comment Organism Structure
additional information use of phosphate buffer yields higher activities than Tris, phthalate, or citrate buffer, optimum concentration is 0.08 - 0.16 M phosphate Aspergillus niger

Application

Application Comment Organism
drug development enzyme could possibly be used as an anti-tumor therapeutic agent Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
Hg2+
-
 Aspergillus niger
Mg2+
-
 Aspergillus niger
additional information no product inhibition Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 L-asparagine pH6, 40°C Aspergillus niger
0.0051
-
 L-glutamine pH6, 40°C Aspergillus niger
2.57
-
 L-asparagine
-
 Aspergillus niger
5.05
-
 L-glutamine
-
 Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-asparagine + H2O Aspergillus niger involved in the maintenance of the correct L-asparagine/L-aspartate ratio L-aspartate + NH3
-
 ?
L-asparagine + H2O Penicillium chrysogenum involved in the maintenance of the correct L-asparagine/L-aspartate ratio L-aspartate + NH3
-
 ?
L-asparagine + H2O Penicillium palitans involved in the maintenance of the correct L-asparagine/L-aspartate ratio L-aspartate + NH3
-
 ?
L-asparagine + H2O Aspergillus niger NRRL 3 involved in the maintenance of the correct L-asparagine/L-aspartate ratio L-aspartate + NH3
-
 ?
L-glutamine + H2O Aspergillus niger involved in the maintenance of the correct L-glutamine/L-glutamate ratio L-glutamate + NH3
-
 ?
L-glutamine + H2O Penicillium chrysogenum involved in the maintenance of the correct L-glutamine/L-glutamate ratio L-glutamate + NH3
-
 ?
L-glutamine + H2O Penicillium palitans involved in the maintenance of the correct L-glutamine/L-glutamate ratio L-glutamate + NH3
-
 ?
L-glutamine + H2O Aspergillus niger NRRL 3 involved in the maintenance of the correct L-glutamine/L-glutamate ratio L-glutamate + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
 NRRL3
-
Aspergillus niger
-
 highest activity in 4 days old nitrate-grown mats
-
Aspergillus niger NRRL 3
-
 NRRL3
-
Aspergillus niger NRRL 3
-
 highest activity in 4 days old nitrate-grown mats
-
Penicillium chrysogenum
-
-
-
Penicillium chrysogenum
-
 nitrate-grown mat
-
Penicillium palitans
-
-
-
Penicillium palitans
-
 nitrate-grown mat
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Storage Stability

Storage Stability Organism
activity is retained in the freezer for some months Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-asparagine + H2O
-
 Aspergillus niger D-aspartate + NH3
-
 ?
L-asparagine + H2O involved in the maintenance of the correct L-asparagine/L-aspartate ratio Aspergillus niger L-aspartate + NH3
-
 ?
L-asparagine + H2O involved in the maintenance of the correct L-asparagine/L-aspartate ratio Penicillium chrysogenum L-aspartate + NH3
-
 ?
L-asparagine + H2O involved in the maintenance of the correct L-asparagine/L-aspartate ratio Penicillium palitans L-aspartate + NH3
-
 ?
L-asparagine + H2O ratio of glutaminase to aspariginase activity is 0.7 to 1 Aspergillus niger L-aspartate + NH3
-
 ?
L-asparagine + H2O ratio of glutaminase to aspariginase activity is 0.8 to 1 Penicillium palitans L-aspartate + NH3
-
 ?
L-asparagine + H2O ratio of glutaminase to aspariginase activity is 1.4 to 1 Penicillium chrysogenum L-aspartate + NH3
-
 ?
L-asparagine + H2O involved in the maintenance of the correct L-asparagine/L-aspartate ratio Aspergillus niger NRRL 3 L-aspartate + NH3
-
 ?
L-glutamine + H2O
-
 Aspergillus niger L-glutamate + NH3
-
 ?
L-glutamine + H2O involved in the maintenance of the correct L-glutamine/L-glutamate ratio Aspergillus niger L-glutamate + NH3
-
 ?
L-glutamine + H2O involved in the maintenance of the correct L-glutamine/L-glutamate ratio Penicillium chrysogenum L-glutamate + NH3
-
 ?
L-glutamine + H2O involved in the maintenance of the correct L-glutamine/L-glutamate ratio Penicillium palitans L-glutamate + NH3
-
 ?
L-glutamine + H2O ratio of glutaminase to aspariginase activity is 0.7 to 1 Aspergillus niger L-glutamate + NH3
-
 ?
L-glutamine + H2O ratio of glutaminase to aspariginase activity is 0.8 to 1 Penicillium palitans L-glutamate + NH3
-
 ?
L-glutamine + H2O ratio of glutaminase to aspariginase activity is 1.4 to 1 Penicillium chrysogenum L-glutamate + NH3
-
 ?
L-glutamine + H2O
-
 Aspergillus niger NRRL 3 L-glutamate + NH3
-
 ?
L-glutamine + H2O involved in the maintenance of the correct L-glutamine/L-glutamate ratio Aspergillus niger NRRL 3 L-glutamate + NH3
-
 ?
L-glutamine + H2O ratio of glutaminase to aspariginase activity is 0.7 to 1 Aspergillus niger NRRL 3 L-glutamate + NH3
-
 ?
additional information acetamide, nicotinamide, and NAD are not hydrolyzed Aspergillus niger ?
-
 ?
additional information acetamide, nicotinamide, and NAD are not hydrolyzed Penicillium chrysogenum ?
-
 ?
additional information acetamide, nicotinamide, and NAD are not hydrolyzed Penicillium palitans ?
-
 ?
additional information acetamide, nicotinamide, and NAD are not hydrolyzed Aspergillus niger NRRL 3 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Penicillium chrysogenum
60
-
-
 Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 80
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, about 10% loss of activity Aspergillus niger
50
-
 30 min, about 40% loss of activity Aspergillus niger
60
-
 30 min, about 70% loss of activity Aspergillus niger
60
-
 incubation at 60°C in the absence of substrate for 20 min causes a 70% increase in activity Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 activity is higher in phosphate buffer than in citrate buffer Aspergillus niger
6 7
-
 Penicillium chrysogenum
6
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
4 9
-
 Aspergillus niger