Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transposon mutagenesis with self-cloning promoter probe vector Tn5-OT182 for inactivation by disruption of gene gltB, gltB mutants lose the ability to utilize amino acids as carbon source, inactivation of gene gltB and other glutamate-induced genes by random mutagenesis, phenotype, overview | Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Pseudomonas putida | - |
- |
periplasm | a functional gltB gene (encoding a subunit of EC 1.4.1.13) is essential for utilization of acidic amino acids and expression of periplasmic glutaminase/asparaginase | Pseudomonas putida | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Pseudomonas putida | - |
L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | Pseudomonas putida KT 2240 | - |
L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | Pseudomonas putida | - |
L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | Pseudomonas putida KT 2240 | - |
L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida | - |
gene gltB | - |
Pseudomonas putida KT 2240 | - |
- |
- |
Pseudomonas putida KT 2240 | - |
gene gltB | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the organism is able to grow on asparagine and glutamine as sole carbon and nitrogen source | Pseudomonas putida | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
intracellular amino acid levels of wild-type and mutant strains | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | - |
Pseudomonas putida | L-aspartate + NH3 | - |
? | |
L-asparagine + H2O | - |
Pseudomonas putida KT 2240 | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | - |
Pseudomonas putida | L-glutamate + NH3 | - |
? | |
L-glutamine + H2O | - |
Pseudomonas putida KT 2240 | L-glutamate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glutaminase/asparaginase | - |
Pseudomonas putida |
periplasmic glutaminase/asparaginase | - |
Pseudomonas putida |
PGA | - |
Pseudomonas putida |