Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoate | complete inhibition at 0.1 mM, asparaginases A and B | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
L-asparagine | asparaginase A | Citrobacter freundii | |
1.7 | - |
L-asparagine | asparaginase B | Citrobacter freundii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Citrobacter freundii |
140000 | - |
asparaginase A, gel filtration | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O | Citrobacter freundii | - |
L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | Citrobacter freundii | low activity | L-glutamate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
3 forms: asparaginase-glutaminase, asparaginase A, asparaginase B | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, asparaginase A | Citrobacter freundii |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Citrobacter freundii | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.6 | - |
asparaginase B | Citrobacter freundii |
133 | - |
asparaginase A | Citrobacter freundii |
Storage Stability | Organism |
---|---|
4°C, 12 months, asparaginase A, asparaginase B: DTT required, 6 months | Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
aspartic acid + H2O | very low activity only for asparaginase B | Citrobacter freundii | ? | - |
? | |
beta-D-aspartyl hydroxylammonium sulfate + H2O | only asparaginase A, low activity | Citrobacter freundii | L-Asp + hydroxylammonium sulfate | - |
? | |
beta-L-aspartyl hydroxylammonium sulfate + H2O | higher activity for asparaginase B | Citrobacter freundii | L-Asp + hydroxylammonium sulfate | - |
? | |
D-asparagine + H2O | low activity | Citrobacter freundii | D-aspartate + NH3 | - |
? | |
D-glutamine + H2O | low activity | Citrobacter freundii | D-glutamate + NH3 | - |
? | |
gamma-L-glutamyl hydroxylammonium sulfate + H2O | very low activities for both asparaginases A and B | Citrobacter freundii | L-glutamate + hydroxylammonium sulfate | - |
? | |
L-asparagine + H2O | - |
Citrobacter freundii | L-aspartate + NH3 | - |
? | |
L-glutamine + H2O | low activity | Citrobacter freundii | L-glutamate + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 33000, SDS-PAGE | Citrobacter freundii |
Synonyms | Comment | Organism |
---|---|---|
asparaginase A | - |
Citrobacter freundii |
asparaginase B | - |
Citrobacter freundii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51 | - |
asparaginase B, half-life: 10 min | Citrobacter freundii |