Any feedback?
Literature summary for 3.5.1.38 extracted from
- Characterization of an extracellular asparaginase of Rhodosporidium toruloides CBS14 exhibiting unique physicochemical properties (1996), Can. J. Microbiol., 42, 316-325.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | slight activation | Rhodotorula toruloides |  |
| cysteine | slight activation | Rhodotorula toruloides | |
| dithiothreitol | slight activation | Rhodotorula toruloides | |
General Stability
| General Stability | Organism |
|---|---|
| stabilizing effect by addition of glycine and maintaining the pH to 9.0 | Rhodotorula toruloides |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-Hydroxy-5-nitrobenzyl bromide | 40% inhibition at 10 mM, concentration-dependent, protection against inhibition in presence of substrate | Rhodotorula toruloides | |
| Hg2+ | slight inhibition | Rhodotorula toruloides | |
| iodoacetamide | slight inhibition | Rhodotorula toruloides | |
| N-bromosuccinimide | concentration-dependent inhibition, 40% at 10 mM, protection against inhibition in presence of substrate | Rhodotorula toruloides | |
| p-chloromercuribenzoate | slight inhibition | Rhodotorula toruloides | |
| Urea | 95% loss of activity in 2.25 M | Rhodotorula toruloides | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.43 | - |
L-asparagine | - |
Rhodotorula toruloides | |
| 2.4 | - |
N-glycyl-L-asparagine | - |
Rhodotorula toruloides | |
| 6.45 | - |
L-glutamine | - |
Rhodotorula toruloides | |
| 8 | - |
N-carbamoyl-L-asparagine | - |
Rhodotorula toruloides | |
| 10 | - |
N-acetyl-L-asparagine | - |
Rhodotorula toruloides | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasmic space | - |
Rhodotorula toruloides | 42597 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni2+ | slightly enhanced activity | Rhodotorula toruloides | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 87000 | - |
2 * 87000, SDS-PAGE | Rhodotorula toruloides |
| 180000 | - |
gel filtration | Rhodotorula toruloides |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-asparagine + H2O | Rhodotorula toruloides | - |
L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | Rhodotorula toruloides | one fifth of the activity on L-asparagine | L-glutamate + NH3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodotorula toruloides | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography, preparative gel electrophoresis | Rhodotorula toruloides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | - |
Rhodotorula toruloides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2156 | - |
glutaminase activity | Rhodotorula toruloides |
| 10510 | - |
asparaginase activity | Rhodotorula toruloides |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10°C, lyophilized to dryness in the presence of 50 mM Tris, 250 mM glycine, pH 9.0, 90% activity in 3 months | Rhodotorula toruloides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-L-aspartyl hydroxylammonium sulfate + H2O | - |
Rhodotorula toruloides | L-Asp + hydroxylammonium sulfate | - |
? | |
| gamma-L-glutamyl hydroxylammonium sulfate + H2O | - |
Rhodotorula toruloides | L-glutamate + hydroxylammonium sulfate | - |
? | |
| L-asparagine + H2O | - |
Rhodotorula toruloides | L-aspartate + NH3 | - |
? | |
| L-asparagine + H2O | not active on D-asparagine | Rhodotorula toruloides | L-aspartate + NH3 | - |
? | |
| L-glutamine + H2O | one fifth of the activity on L-asparagine | Rhodotorula toruloides | L-glutamate + NH3 | - |
? | |
| N-acetyl-L-asparagine + H2O | - |
Rhodotorula toruloides | N-acetyl-L-aspartate + NH3 | - |
? | |
| N-carbamoyl-L-asparagine + H2O | - |
Rhodotorula toruloides | N-carbamoyl-L-aspartate + NH3 | - |
? | |
| N-glycyl-L-asparagine + H2O | 60% higher activity than on L-asparagine | Rhodotorula toruloides | N-glycyl-L-aspartate + NH3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 87000, SDS-PAGE | Rhodotorula toruloides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Rhodotorula toruloides |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 7 | optimal range for asparaginase activity | Rhodotorula toruloides |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
complete inactivation | Rhodotorula toruloides |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
