Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Citrobacter freundii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP | at pH 7.0, temperature not specified in the publication | Citrobacter freundii | |
0.5 | - |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | at pH 7.0, temperature not specified in the publication | Citrobacter freundii | |
1.76 | - |
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | at pH 7.0, temperature not specified in the publication | Citrobacter freundii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP + H2O | Citrobacter freundii | - |
1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP | - |
? | |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O | Citrobacter freundii | - |
1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | - |
? | |
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O | Citrobacter freundii | the substrate is likely not turned over in vitro by AmpD | ? | - |
? | |
bacterial peptidoglycan + H2O | Citrobacter freundii | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrobacter freundii | - |
strain ATCC6879 | - |
Purification (Comment) | Organism |
---|---|
- |
Citrobacter freundii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP + H2O | - |
Citrobacter freundii | 1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP | - |
? | |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O | - |
Citrobacter freundii | 1,6-anhydro-MurNAc + L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | - |
? | |
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala + H2O | the substrate is likely not turned over in vitro by AmpD | Citrobacter freundii | ? | - |
? | |
bacterial peptidoglycan + H2O | - |
Citrobacter freundii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmpD | - |
Citrobacter freundii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | at pH 7.0, temperature not specified in the publication | Citrobacter freundii | |
11 | - |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | at pH 7.0, temperature not specified in the publication | Citrobacter freundii | |
25 | - |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP | at pH 7.0, temperature not specified in the publication | Citrobacter freundii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
4-O-beta-D-GlcNAc-1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | at pH 7.0, temperature not specified in the publication | Citrobacter freundii | |
22 | - |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP-D-Ala-D-Ala | at pH 7.0, temperature not specified in the publication | Citrobacter freundii | |
69 | - |
1,6-anhydro-MurNAc-L-Ala-gamma-D-Glu-m-DAP | at pH 7.0, temperature not specified in the publication | Citrobacter freundii |