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Literature summary for 3.5.1.2 extracted from

  • Pu, H.; Wang, Q.; Zhu, F.; Cao, X.; Xin, Y.; Luo, L.; Yin, Z.
    Cloning, expression of glutaminase from Pseudomonas nitroreducens and application to theanine synthesis (2013), Biocatal. Biotransform., 31, 1-7.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pseudomonas nitroreducens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
93500
-
x * 93500, calculated from amino acid sequence Pseudomonas nitroreducens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamine + H2O Pseudomonas nitroreducens
-
L-glutamate + NH3
-
?
L-glutamine + H2O Pseudomonas nitroreducens DSM 14399
-
L-glutamate + NH3
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas nitroreducens K7NB71
-
-
Pseudomonas nitroreducens DSM 14399 K7NB71
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Pseudomonas nitroreducens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-gamma-glutamyl-4-nitroanilide + H2O
-
Pseudomonas nitroreducens L-glutamate 4-nitroanilide + NH3
-
?
L-gamma-glutamyl-4-nitroanilide + H2O
-
Pseudomonas nitroreducens DSM 14399 L-glutamate 4-nitroanilide + NH3
-
?
L-glutamine + ethylamine
-
Pseudomonas nitroreducens theanine + NH3
-
?
L-glutamine + ethylamine
-
Pseudomonas nitroreducens DSM 14399 theanine + NH3
-
?
L-glutamine + H2O
-
Pseudomonas nitroreducens L-glutamate + NH3
-
?
L-glutamine + H2O
-
Pseudomonas nitroreducens DSM 14399 L-glutamate + NH3
-
?

Subunits

Subunits Comment Organism
? x * 93500, calculated from amino acid sequence Pseudomonas nitroreducens

Synonyms

Synonyms Comment Organism
GLS
-
Pseudomonas nitroreducens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Pseudomonas nitroreducens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 42 about 50% activity at 30°C, about 60% activity at 32°C, about 80% activity at 35°C, 100% activity at 37°C, about 75% activity at 40°C, about 55% activity at 42°C Pseudomonas nitroreducens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Pseudomonas nitroreducens

pH Range

pH Minimum pH Maximum Comment Organism
8 12 about 85% activity at pH 8.0, about 90% activity at pH 9.0, 100% activity at pH 10.0, about 45% activity at pH 11.0, about 30% activity at pH 12.0 Pseudomonas nitroreducens

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas nitroreducens calculated from amino acid sequence
-
6.8